Oviductus ranae (O.ran.) has been widely used as a tonic and a traditional animal-based Chinese medicine. O.ran. extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic. Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities. However, their presence and possible structural characteristics within O.ran. were previously unknown. In this study, glycosaminoglycans were isolated from O.ran. and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran. with varied disaccharide compositions. HS species contain highly acetylated disaccharides, and have various structures in their constituent chains. CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran..

牛输卵管( O.ran . ) 已被广泛用作滋补品和传统的动物性中药。欧然。据报道，提取物具有多种生物活性，包括通常与哺乳动物糖胺聚糖相关的活性，例如抗炎、抗骨质疏松和抗哮喘。糖胺聚糖是哺乳动物中普遍存在的复杂线性多糖，具有广泛的生物活性。然而，它们在O.ran 中的存在和可能的结构特征。以前是未知的。在这项研究中，糖胺聚糖是从O.ran 中分离出来的。并通过液相色谱-离子阱/飞行时间质谱（LC-MS-ITTOF）分析它们的二糖组成。在O.ran中检测到硫酸乙酰肝素(HS)/肝素(HP)、硫酸软骨素(CS)/硫酸皮肤素(DS)和透明质酸(HA) 。具有不同的二糖成分。HS 物种含有高度乙酰化的二糖，并在其组成链中具有各种结构。CS/DS 链还具有异质结构，具有不同的硫酸化模式和密度。这种新颖的结构信息有助于阐明这些多糖可能参与O.ran 的生物活性。.