Autolytic debridement can accelerate wound healing by removing necrotic tissue. A hydrogel was fabricated from an aqueous solution of gelatin, sodium alginate and carboxymethylcellulose sodium by radiation-induced cross-linking at room temperature, which was aiming at the application of debridement glue paste. The swelling ratio of the debridement glue paste is 30 times to its dry weight, when the weight ratio of gelatin/sodium alginate/carboxymethylcellulose sodium was 2:2:2 and the absorbed dose was 20 kGy, with dose rate of 20 Gy/min. The extrusion and compressive assay have confirmed that it possessed stable mechanical strength, and the weight ratio had little effect on the molecular structure by FTIR and TGA. Cell culture experiments demonstrated the debridement glue pastes with the cytotoxicity of grade 0 or 1 (biosafe). The debridement glue paste group could remove the necrotic tissue within 4 days and showed complete wound healing within 18 days; comparatively, the control group without treatment removed the necrotic tissue within 10 days and showed complete wound healing within 26 days in animal experiments using rabbit scald model. Histologic analysis exhibited that more granulation tissue was observed in debridement glue paste. The result of this study suggested that debridement glue pastes had excellent biocompatibility, could selectively remove necrotic tissue, induced granulation tissue formation and accelerated the wound healing.

中文翻译：

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辐射交联明胶/海藻酸钠/羧甲基纤维素钠水凝胶作为清创胶膏的应用

自溶性清创可以通过去除坏死组织来加速伤口愈合。以明胶、海藻酸钠和羧甲基纤维素钠的水溶液为原料，在室温下通过辐射诱导交联制备水凝胶，用于清创胶膏的应用。当明胶/海藻酸钠/羧甲基纤维素钠的重量比为2:2:2，吸收剂量为20 kGy，剂量率为20 Gy/min时，清创胶膏的溶胀比为干重的30倍. 挤压和压缩试验证实，它具有稳定的机械强度，重量比对FTIR和TGA的分子结构影响不大。细胞培养实验表明，清创胶膏具有 0 级或 1 级（生物安全）的细胞毒性。清创胶贴组可在4天内清除坏死组织，18天内创面完全愈合；相比之下，在兔烫伤模型的动物实验中，未治疗的对照组在10天内去除了坏死组织，并在26天内伤口完全愈合。组织学分析表明，在清创胶糊中观察到更多的肉芽组织。本研究结果表明，清创胶膏具有优异的生物相容性，可选择性去除坏死组织，诱导肉芽组织形成，加速创面愈合。对照组未经处理，10天内去除坏死组织，26天内创面完全愈合，兔烫伤模型动物实验。组织学分析表明，在清创胶糊中观察到更多的肉芽组织。本研究结果表明，清创胶膏具有优异的生物相容性，可选择性去除坏死组织，诱导肉芽组织形成，加速创面愈合。对照组在10天内去除坏死组织，在兔烫伤模型动物实验中26天内伤口完全愈合。组织学分析表明，在清创胶糊中观察到更多的肉芽组织。本研究结果表明，清创胶膏具有优异的生物相容性，可选择性去除坏死组织，诱导肉芽组织形成，加速创面愈合。