Plants develop a series of physiological, biochemical, and molecular responses to survive in drought stress. Many drought-related genes with various functions which regulated by ABA-dependent and independent pathways have been identified in wheat. However, studies on the effect of biostimulants on drought-related genes are limited and unclear. As a biostimulant, humic acid (HA) has enormous roles on plant growth, yield, and protection of resistance in various abiotic stresses, but its relation to drought-related genes is unknown. Here, we aimed to determine the relationships between drought stress generated by − 6 bar and − 8 bar PEG6000 and ABA and HA treatments on total oxidant status (TOS) and total antioxidant status (TAS) and expression of miRNA target genes (AP2, GRF, LAC, CSD1/CSD2, and Plastocyanin) on tolerant (Aksel 2000 and Kırkpınar 79) and susceptible (Atlı 2002 and Kırmızı Kılçık) bread wheat (Triticum aestivum L.) genotypes. According to the results, the expression of the genes varied depending on genotype and tissue and it was determined whether the genes used ABA-dependent or ABA-independent mechanisms to provide tolerance to stress in our promoter analysis. Also, the irregularities of the biochemical changes and expression of genes that were caused by PEG6000 could be improved by HA considering the positive effects on genes.

植物会发展出一系列生理，生化和分子反应，以在干旱胁迫下生存。在小麦中已经鉴定出许多干旱相关基因，这些基因具有受ABA依赖和独立途径调控的各种功能。但是，关于生物刺激物对干旱相关基因的影响的研究是有限的，还不清楚。作为一种生物刺激剂，腐殖酸（HA）在植物的生长，产量以及在各种非生物胁迫下的抗性保护方面均发挥着巨大作用，但其与干旱相关基因的关系尚不清楚。在这里，我们旨在确定− 6 bar和− 8 bar PEG6000和ABA和HA处理产生的干旱胁迫与总氧化剂状态（TOS）和总抗氧化剂状态（TAS）以及miRNA靶基因（AP2，GRF）表达之间的关系。 ，LAC，CSD1 / CSD2，普通小麦基因型）。根据结果​​，基因的表达随基因型和组织的不同而变化，并且在我们的启动子分析中确定了基因是使用ABA依赖性还是ABA依赖性机制来提供对压力的耐受性。此外，考虑到对基因的积极影响，HA可以改善由PEG6000引起的生化变化和基因表达的不规则性。