This study reports the sugaring-out extraction of erythromycin from fermentation broth using acetonitrile (ACN) as solvent and glucose as a mass separating agent. Different process parameters—glucose concentration, temperature, ACN/water ratio and pH—were optimized to achieve maximum extraction of erythromycin. 88% (w/w) of erythromycin was extracted from the model system with following optimized conditions: glucose 156.3 g/L; temperature 4 °C; ACN/water ratio 1 and pH 8.3. Further, the effect of typical fermentation media components (starch, soybean flour, CaCO 3 , NaCl and (NH 4 ) 2 SO 4 ) on sugaring out extraction of erythromycin was also investigated. Starch, soybean flour and CaCO 3 were observed to affect erythromycin extraction only at higher concentration. Removal of suspended solids from simulated as well as real broth prior to extraction enhanced the extraction efficiency (from 72% to 87%). Sugaring out extraction of erythromycin was found to be more effective than salting out extraction. Also, higher partition coefficient was achieved in the present work than other reported methods using carbohydrates as mass separating agent. Further, it was found that the antimicrobial activity of erythromycin was preserved during sugaring out extraction of erythromycin.

中文翻译：

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从发酵液中糖析提取红霉素


本研究报告了使用乙腈（ACN）作为溶剂和葡萄糖作为质量分离剂从发酵液中糖化提取红霉素。对不同的工艺参数（葡萄糖浓度、温度、乙腈/水比例和 pH 值）进行了优化，以实现红霉素的最大提取。在以下优化条件下，从模型系统中提取了 88%（w/w）的红霉素：葡萄糖 156.3 g/L；温度4℃； ACN/水比例为 1，pH 为 8.3。此外，还研究了典型发酵培养基成分(淀粉、大豆粉、CaCO 3 、NaCl和(NH 4 ) 2 SO 4 )对红霉素糖化提取的影响。观察到淀粉、大豆粉和CaCO 3 仅在较高浓度时影响红霉素提取。在提取之前去除模拟肉汤和真实肉汤中的悬浮固体提高了提取效率（从 72% 到 87%）。发现红霉素的糖析提取比盐析提取更有效。此外，与其他报道的使用碳水化合物作为质量分离剂的方法相比，本工作实现了更高的分配系数。此外，还发现红霉素的抗微生物活性在红霉素的加糖提取过程中得以保留。