Bio-energy crops need to be grown on marginal salt and drought lands in India as per policy. Understanding environmental stress response in bio-energy crops might help in promoting cultivation of bio-energy feedstock on marginal salty and drought land. This is one of the first report for vegetative propagation of Bamboo (Bambusa balcooa) under salt and drought stress to understand antioxidant enzymes’ gene regulations to combat stress through activation of antioxidant enzymes and osmo-protectant molecules to scavenge reactive oxygen species as measured by physiological changes. Morphological, physiological, and biochemical traits were noted as indicators of plant health upon different sodium chloride (NaCl) salt-stress while various drought conditions with correlation analysis. A significant up-regulation of genes related to most of the antioxidant enzymes was observed up to salinity of 14 mS cm^− 1 electric conductivity (EC) at 150 mM NaCl experimental salt stress which declined with higher salt-stress. While in the case of drought-stress, all genes remained up-regulated while proline dehydrogenase (PDH) remained down-regulated up-to 100% drought-stress having 4% soil moisture. The gene expressions of antioxidant enzymes were significantly correlated with their corresponding gene-products namely super-oxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and ascorbate peroxidase (APX) activities. Biochemical parameters such as, soluble sugar, proline, malondialdehyde (MDA), total amino acids, hydrogen peroxide and electrolyte leakage ratio also showed positive correlation (p = 0.001) with salt condition. Genetic and biochemical test parameters were significantly correlated with physiological attributes of plant health at soil EC of 14 mS cm^− 1 shown as 150 mM NaCl salt stress and 60% drought-stress having 17% soil moisture content, were the optimum stress tolerance limits observed. Application of these data would be useful to cultivate 0.63 million ha of salinity affected land and 10.05 million ha of drought affected land among wastelands in India to meet biofuel need.

根据政策，生物能源作物需要在印度的边际盐碱地和干旱土地上种植。了解生物能源作物的环境胁迫反应可能有助于促进在边缘盐碱地和干旱土地上种植生物能源原料。这是关于竹子（ Bambusa balcooa）营养繁殖的第一份报告在盐和干旱胁迫下了解抗氧化酶的基因调控，通过激活抗氧化酶和渗透保护分子来清除通过生理变化测量的活性氧来对抗压力。形态学、生理学和生化性状被认为是不同氯化钠 (NaCl) 盐胁迫下植物健康的指标，同时具有相关性分析的各种干旱条件。^{在高达 14 mS cm − 1}的盐度下，观察到与大多数抗氧化酶相关的基因显着上调150 mM NaCl 实验盐胁迫下的电导率 (EC) 随着盐胁迫的升高而下降。而在干旱胁迫的情况下，所有基因都保持上调，而脯氨酸脱氢酶 (PDH) 保持下调，直至 100% 干旱胁迫具有 4% 的土壤水分。抗氧化酶的基因表达与其相应的基因产物显着相关，即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和抗坏血酸过氧化物酶（APX）活性。可溶性糖、脯氨酸、丙二醛（MDA）、总氨基酸、过氧化氢和电解质渗漏率等生化参数也呈正相关（p = 0.001) 含盐条件。^{遗传和生化测试参数与土壤 EC 为 14 mS cm - 1}的植物健康生理属性显着相关，显示为 150 mM NaCl 盐胁迫和具有 17% 土壤水分含量的 60% 干旱胁迫，是观察到的最佳胁迫耐受限度. 这些数据的应用将有助于在印度荒地中种植 63 万公顷受盐碱影响的土地和 1005 万公顷受干旱影响的土地，以满足生物燃料的需求。