Laurencia catarinensis (Ceramiales, Rhodomelaceae) has a high diversity of secondary metabolites such as acetogenin and halogenated terpenoids, with biological activities. We tested different combinations of auxins and cytokinins to evaluate their effects on the growth, total soluble proteins, pigments, and carbohydrates of L. catarinensis with the aim of improving the culture techniques of this species. To accomplish this, algae were cultivated in seawater enriched with quarter strength of von Stosch solution (VSES/4) with the addition of plant growth regulators (PGRs), and the treatment control without PGRs addition. The auxins tested were indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D), and cytokinins were benzylaminopurine (BA) and 2-isopentenyladenine (2iP), in the following combinations: IAA:BA, IAA:2iP, 2,4-D:BA, and 2,4-D:2iP, in the concentrations of 5:5, 5:50, 50:5, and 50:50 μM for each auxin:cytokinin combination. Addition of PGRs had significant effects on the growth rates of L. catarinensis, which varied from 7.62 ± 0.12% to 9.30 ± 0.55% day⁻¹. The highest growth rates were observed in treatments with 5:5 μM of IAA:BA and 2,4-D:BA, and 50:5 μM of 2,4-D:2iP. Higher contents of total soluble proteins were observed in treatments with 50:50 μM of IAA:BA and 2,4-D:BA. The auxin:cytokinin combinations inhibited allophycocyanin contents, but did not affect chlorophyll a and carbohydrate concentrations. Therefore, supplementation of auxins and cytokinins, especially the IAA:BA and 2,4-D:BA combinations, is useful approach to stimulate growth rates or soluble protein contents of L. catarinensis, improving the cultivation of this species with high biotechnological potential.

Laurencia catarinensis（Ceramiales，Rhodomelaceae）具有多种多样的次生代谢产物，例如产黄素和卤代萜类，具有生物活性。我们测试了生长素和细胞分裂素的不同组合，以评估它们对卡塔氏乳杆菌生长，总可溶性蛋白，色素和碳水化合物的影响旨在改善该物种的养殖技术。为此，在添加了植物生长调节剂（PGR）的情况下，在富含四分之一冯·斯托施溶液（VSES / 4）的海水中培养藻类，并在不添加PGR的情况下进行处理控制。测试的生长素是吲哚-3-乙酸（IAA）和2,4-二氯苯氧基乙酸（2,4-D），而细胞分裂素是苄氨基嘌呤（BA）和2-异戊烯腺嘌呤（2iP），以下组合：IAA：生长素：细胞分裂素组合的BA，IAA：2iP，2,4-D：BA和2,4-D：2iP的浓度为5：5、5：50、50：5和50:50μM 。PGR的添加对卡塔氏乳杆菌的生长速率有显着影响，从第^-1天的7.62±0.12％到9.30±0.55％不等。在使用5：5μM的IAA：BA和2,4-D：BA和50：5μM的2,4-D：2iP的处理中观察到最高的生长速率。在使用50:50μM的IAA：BA和2,4-D：BA的处理中，观察到总可溶性蛋白含量更高。生长素：细胞分裂素的组合抑制了藻蓝蛋白的含量，但不影响叶绿素a和碳水化合物的浓度。因此，补充植物生长素和细胞分裂素，特别是IAA：BA和2,4-D：BA组合，是刺激卡塔氏乳杆菌生长速率或可溶性蛋白含量的有用方法，可提高具有高生物技术潜力的该物种的培养。