Biogenic silver nanoparticles (AgNPs) are of significant interest due to their widespread application. In order to make nontoxic cost-effective AgNPs, several microorganisms have been utilized. In the present study, emphasis was given to Anabaena variabilis–mediated AgNPs synthesis. Optimal conditions for extract preparation were heating of extraction mixture at 100 °C for 5 min, while for AgNPs synthesis, optimal conditions were 1:9 v/v (cell extract: AgNO₃ (1 mM)), pH 7.4, and 30 °C reaction temperatures. The synthesized AgNPs were characterized initially by UV-Vis spectrophotometer and the maximum absorbance peak was obtained at 440 nm. XRD technique confirmed their crystalline nature. SEM with EDX showed 66% elemental silver by weight, which signified the purity of AgNPs. DLS study showed nearly monodispersed nature of AgNPs. TEM observation confirmed the synthesis of 11–15 nm spherical NPs. FTIR analysis of synthesized AgNPs showed the functional moieties responsible for their bioactivity. The biomolecules of AgNPs were confirmed by LCMS/MS. Anabaena variabilis–mediated AgNPs showed good antibacterial and antifungal activity. In combination with standard antibiotic (streptomycin) and antifungal agents (amphotericin B, fluconazole), AgNPs showed significant synergistic effect. The blend of antibacterial and antifungal properties, coupled with their intrinsic “green” and facile synthesis, made these biogenic nanoparticles attractive in nanomedicine.

由于生物银纳米颗粒（AgNPs）的广泛应用，它们引起了人们的极大兴趣。为了制造无毒，成本效益高的AgNP，已经使用了几种微生物。在本研究中，重点是变色鱼腥藻介导的AgNPs合成。提取物制备的最佳条件是将提取混合物在100°C下加热5分钟，而AgNPs合成的最佳条件是1：9 v / v（细胞提取物：AgNO ₃（1 mM）），pH 7.4和30°C反应温度。最初通过紫外可见分光光度计对合成的AgNPs进行表征，并在440 nm处获得最大吸收峰。XRD技术证实了其晶体性质。带有EDX的SEM显示重量百分比为66％的元素银，表明AgNP的纯度。DLS研究表明AgNP几乎是单分散的。TEM观察证实了11-15 nm球形NP的合成。合成AgNPs的FTIR分析表明，负责其生物活性的功能部分。AgNP的生物分子通过LCMS / MS确认。鱼腥藻–介导的AgNPs显示出良好的抗菌和抗真菌活性。与标准抗生素（链霉素）和抗真菌药（两性霉素B，氟康唑）联合使用时，AgNPs表现出显着的协同作用。抗菌和抗真菌特性的融合，以及其固有的“绿色”和便捷的合成，使得这些生物纳米颗粒在纳米药物中具有吸引力。