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Effect of light quality and quantity on productivity and phycoerythrin concentration in the cryptophyte Rhodomonas sp .
Journal of Applied Phycology ( IF 2.8 ) Pub Date : 2021-01-07 , DOI: 10.1007/s10811-020-02338-3
Christos Latsos , Jasper van Houcke , Lander Blommaert , Gabrielle P. Verbeeke , Jacco Kromkamp , Klaas R. Timmermans

The cryptophyte Rhodomonas sp. is a potential feed source for aquaculture live feed and resource for phycoerythrin (PE) production. This research investigates the influence of light, both quality and quantity, on the biomass productivity, composition and growth rate of Rhodomonas sp. The incident light intensity used in the experiments was 50 μmolphotons m−2 s−1, irrespective of the colour of the light, and cultivation took place in lab-scale flat-panel photobioreactors in turbidostat mode. The highest productivity in volumetric biomass (0.20 gdry weight L−1 day−1), measured under continuous illumination, was observed under green light conditions. Blue and red light illumination resulted in lower productivities, 0.11 gdry weight L−1 day−1 and 0.02 g L−1 day−1 respectively. The differences in production are ascribed to increased absorption of green and blue wavelength by phycoerythrin, chlorophyll and carotenoids, causing higher photosynthetically usable radiation (PUR) from equal photosynthetically absorbed irradiance (PAR). Moreover, phycoerythrin concentration (281.16 mg gDW−1) was stimulated under red light illumination. Because photosystem II (PSII) absorbs poorly red light, the algae had to induce more pigments in order to negate the lower absorption per unit pigment of the incident available photons. The results of this study indicate that green light can be used in the initial growth of Rhodomonas sp. to produce more biomass and, at a later stage, red light could be implemented to stimulate the synthesis of PE. Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated a significant difference between the cells under different light quality, with higher contents of proteins for samples of Rhodomonas sp. cultivated under green light conditions. In comparison, higher carbohydrate contents were observed for cells that were grown under red and blue light.



中文翻译:

光照质量和数量对隐藻Rhodomonas sp。生产力和藻红蛋白浓度的影响。

植物Rhodomonas sp 是水产养殖活饲料和藻红蛋白(PE)生产资源的潜在饲料来源。这项研究调查了光,质和量对红球藻生物量生产力,组成和生长速率的影响。实验中使用的入射光强度为50μmol光子m -2  s -1,与光的颜色无关,并且以turbidostat模式在实验室规模的平板光生物反应器中进行培养。最高的生物量生产率(0.20 g干重L -1 天-1在绿光条件下观察在连续照明下测得的)。蓝光和红光照射导致生产率降低,分别为0.11 g干重L -1 天-1和0.02 g L -1 天-1。产量上的差异归因于藻红蛋白,叶绿素和类胡萝卜素对绿色和蓝色波长的吸收增加,从而由相等的光合作用吸收辐照度(PAR)引起更高的光合可用辐射(PUR)。此外,藻红蛋白浓度(281.16 mg gDW -1)是在红光照射下刺激的。由于光系统II(PSII)吸收的红光较弱,因此藻类必须诱导更多的色素,以抵消入射的可用光子的每单位色素较低的吸收。这项研究的结果表明,绿光可用于红景天菌的初始生长产生更多的生物质,在以后的阶段,可以实施红光刺激PE的合成。傅里叶变换红外光谱(FTIR)分析表明,在不同光质量下,细胞之间的显着差异,红景天菌样品的蛋白质含量更高在绿灯条件下种植。相比之下,对于在红色和蓝色光下生长的细胞,观察到较高的碳水化合物含量。

更新日期:2021-01-07
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