Cytoplasmic male sterility (CMS) is a widely applied plant breeding tool for hybrid seed production. The phenomenon is often caused by chimeric genes with altered open reading frames (ORFs) located in the mitochondrial genomes and expressed as novel genotoxic products that induce pollen abortion. The fertility of CMS plants can be restored by nuclear-encoded genes that inhibit the action of ORFs responsible for pollen sterility. A recombinant inbred line (RIL) mapping population S64/04/01, encompassing 175 individuals, was used for genetic map construction and identification of quantitative trait loci (QTLs) responsible for fertility restoration in rye (Secale cereale L.) with CMS Pampa. The genetic map of all seven rye chromosomes included 15,516 SNP and silicoDArT markers and covered 1070.5 cm. Individual QTLs explaining 60% and 5.5% of the fertility trait’s phenotypic variance were mapped to chromosomes 4R (QRft-4R) and 5R (QRft-5R), respectively. Association mapping identified markers with the highest R² value of 0.58 (p value = 2.21E-28). Markers showing the highest associations with the trait were also mapped to the 4R chromosome within the QRft-4R region. Based on marker sequence homology, putative genes involved in pollen fertility restoration were suggested. Five silicoDArTs were converted into PCR-based markers for further breeding purposes.

细胞质雄性不育 (CMS) 是一种广泛应用于杂交种子生产的植物育种工具。这种现象通常是由位于线粒体基因组中的具有改变的开放阅读框 (ORF) 的嵌合基因引起的，并表达为诱导花粉流产的新型基因毒性产物。CMS 植物的生育力可以通过核编码基因来恢复，这些基因抑制负责花粉不育的 ORF 的作用。的重组近交系（RIL）作图群体/ 04/01时，包含175名个人，被用于遗传图谱构建和数量性状基因座（QTL）负责育性恢复黑麦（识别S64黑麦L.) 与 CMS Pampa。所有七个黑麦染色体的遗传图谱包括 15,516 个 SNP 和 silicoDArT 标记，覆盖 1070.5 cm。解释生育性状表型变异 60% 和 5.5% 的单个 QTL 分别定位到染色体 4R ( QRft-4R ) 和 5R ( QRft-5R )。关联作图确定了具有最高R ²值 0.58 的标记（p值 = 2.21E-28）。显示与性状最高关联的标记也被定位到QRft-4R区域内的 4R 染色体。基于标记序列的同源性，提出了与花粉育性恢复有关的推定基因。五个 silicoDArT 被转化为基于 PCR 的标记，用于进一步的育种目的。