Tungsten oxide nanoparticles or nanopowder (WO₃NPs) is commonly used in various industries and also in biomedical applications such as additives, pigments, and biomedical sensors. Non-judicious excessive use of these nanoparticles (NPs) could be a serious human health concern. Therefore, the current study aimed to explore the cytotoxic and genotoxic assessment of WO₃NPs through Allium cepa anaphase-telophase and comet assays. Nanoparticles were characterized through the scanning and transmission electron microscopy (TEM), zetasizer, and energy-dispersive X-ray spectroscopy. The mean size and the average diameter of WO₃NPs were determined as 21.57 ± 2.48 nm and 349.42 ± 80.65 nm using TEM and a Zetasizer measurement system, respectively. Five concentrations (12.5 mg/L, 25 mg/L, 50 mg/L, 75 mg/L, and 100 mg/L) of WO₃NPs were employed on the Allium cepa (A. cepa) roots for 4 h. Significant (p ≤ 0.05) decrease in mitotic index (MI) was shown by WO₃NPs at all concentrations. The increase of chromosomal aberrations (CAs) was also observed in a concentration-dependent manner due to the WO₃NPs exposure. There was a significant increase (p ≤ 0.05) in DNA damage at all concentrations of WO₃NPs on the A. cepa cells. It was concluded that WO₃NPs had cytotoxic and genotoxic effects on A. cepa meristematic cells. Moreover, further cytogenetic effects of WO₃NPs should be investigated at the molecular level to assess its safety margin.

氧化钨纳米粒子或纳米粉末 (WO ₃ NPs) 常用于各种行业以及生物医学应用，例如添加剂、颜料和生物医学传感器。不明智地过度使用这些纳米颗粒 (NPs) 可能是一个严重的人类健康问题。因此，目前的研究旨在通过洋葱洋葱后期-末期和彗星试验探索 WO ₃ NPs的细胞毒性和基因毒性评估。通过扫描和透射电子显微镜 (TEM)、zetasizer 和能量色散 X 射线光谱对纳米颗粒进行了表征。WO ₃的平均粒径和平均粒径NPs 分别使用 TEM 和 Zetasizer 测量系统确定为 21.57 ± 2.48 nm 和 349.42 ± 80.65 nm。将五种浓度（12.5 mg/L、25 mg/L、50 mg/L、75 mg/L 和 100 mg/L）的 WO ₃ NPs 用于洋葱( A. cepa ) 根部 4 小时。 WO ₃ NP 在所有浓度下均显示有丝分裂指数 (MI)显着降低( p ≤ 0.05) 。由于 WO ₃ NPs 暴露，还以浓度依赖性方式观察到染色体畸变 (CA) 的增加。 在所有浓度的 WO ₃ NPs 对A. cepa 的DNA 损伤都有显着增加 ( p ≤ 0.05)细胞。结论是WO ₃ NPs对A. cepa分生组织细胞具有细胞毒性和基因毒性作用。此外，应在分子水平上研究WO ₃ NPs 的进一步细胞遗传学效应，以评估其安全范围。