This study was designed to evaluate the possibility of using phage-amplification assay for discriminating between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium. The characteristics of Salmonella phage PBST32 were determined by adsorption rate, one-step growth curve, and lytic activity. The ability of phage-based method to detect S. Typhimurium ATCC 19585 (ST^CIP) was determined in single culture and bacterial mixtures of S. Typhimurium ATCC 19585 (ST^WT), Klebsiella pneumoniae, and Staphylococcus aureus. The adsorption rates of PBST32 were 95% and 93% against ST^WT and ST^CIP after 20 min, respectively. The PBST32 showed latent period of 20 min and average burst size of 90 against ST^WT and ST^CIP. The ST^CIP was selectively detected in mixtures of S. aureus, K. pneumoniae, and ST^WT by phage amplification assay. These results provide useful information for designing phage amplification method that can differentially detect antibiotic-resistant pathogens.

本研究旨在评估使用噬菌体扩增试验区分抗生素敏感和抗生素抗性鼠伤寒沙门氏菌的可能性。沙门氏菌噬菌体PBST32的特性由吸附率、一步生长曲线和裂解活性确定。基于噬菌体的方法来检测的能力S.鼠伤寒沙门氏菌ATCC 19585（ST ^CIP）的混合物在单培养和细菌的混合物确定小号。Typhimurium ATCC 19585 (ST ^WT )、肺炎克雷伯菌和金黄色葡萄球菌。PBST32 对 ST ^WT和 ST的吸附率为 95% 和 93%分别在 20 分钟后进行^CIP。PBST32 对 ST ^WT和 ST ^{CIP 的}潜伏期为 20 分钟，平均爆发大小为 90 。ST ^CIP在S 的混合物中被选择性地检测到。金黄色葡萄球菌，ķ。通过噬菌体扩增试验检测肺炎链球菌和 ST ^WT。这些结果为设计可以差异检测抗生素抗性病原体的噬菌体扩增方法提供了有用的信息。