The use of dietary polyphenols as antimicrobial agents has gained immense popularity in recent years, although few of them-like tannic acid has limited use in this field of research; one of the main reasons is its restricted access through the bacterial membrane. Dissipating the bacterial membrane potential with a sub-lethal dosage of the protonophore, carbonyl cyanide m-chlorophenyl hydrazone, enhanced the tannic acid-cytotoxicity with subsequent inhibition of aerobic respiration in Pseudomonas aeruginosa strains which otherwise exhibited a minimum response to tannic acid. However, ascorbic acid, an antioxidant and bacterial membrane-stabilizing compound, had rescued the cells from both tannic acid- and CCCP-mediated lethality. The results suggested that dispersing the membrane potential with a protonophore can enhance the antibacterial properties of tannic acid.

中文翻译：

---

膜去极化使铜绿假单胞菌对单宁酸敏感

近年来，膳食多酚作为抗微生物剂的使用广受欢迎，尽管其中很少有类似单宁酸在该研究领域的应用受到限制。主要原因之一是它通过细菌膜的进入受到限制。用亚致死剂量的质子载体、羰基氰化物间氯苯腙消散细菌膜电位，增强了单宁酸的细胞毒性，随后抑制了铜绿假单胞菌菌株的有氧呼吸，否则铜绿假单胞菌对单宁酸的反应最小。然而，抗坏血酸，一种抗氧化剂和细菌膜稳定化合物，已经从单宁酸和 CCCP 介导的致死率中拯救了细胞。