Purpose

CMV antigens have been detected in some brain tumors specially glioblastoma multiforme (GBM). As brain tumors in the first years of life are among the most aggressive neoplasms with poor prognosis, novel therapeutic options like targeted therapy against virus antigens are demanded. Infantile central nervous system tumors, other than GBM, have not been so far studied for CMV. To our best knowledge, this is the first study in which the presence of CMV-DNA, as a potential viral target for therapy, in non-GBM infantile brain tumors has been investigated.

Methods

The paraffin blocks of non-GBM brain neoplasms of 36 infants (age < 24 months) who were operated on between 2006 and 2016 were examined for CMV-DNA, using real-time polymerase chain reaction (PCR). Paraffin blocks of CMV infected lung tissue were used as positive control. Extraction and amplification of β2 microglobulin gene from each tumor tissue were carried as positive internal control. We also assayed 25 paraffin blocks of meningomyelocele for CMV DNA as negative tissue controls.

Results

Histopathological diagnoses consisted of 13 glial/neuroglial tumors (36.1%), 8 ependymomas (22.2%), 7 medulloblastomas (19.4%), 3 choroid plexus tumors (8.3%), 2 atypical teratoid rhabdoid tumors (5.6%), 2 embryonal CNS tumors (5.6%), and 1 germ cell tumor (2.8%). We could not detect CMV DNA in all samples examined.

Conclusion

Although CMV may be associated with GBM, no role could be proposed for this virus in development of non-GBM infantile brain tumors. Further investigations on larger series of brain tumors should be conducted to confirm or rule out our conclusion.

中文翻译：

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婴儿非胶质母细胞瘤多形性脑肿瘤中的巨细胞病毒 DNA

目的

在一些脑肿瘤中检测到 CMV 抗原，特别是多形性胶质母细胞瘤 (GBM)。由于生命最初几年的脑肿瘤是预后最差的最具侵袭性的肿瘤之一，因此需要新的治疗选择，例如针对病毒抗原的靶向治疗。除 GBM 外，婴儿中枢神经系统肿瘤迄今尚未针对 CMV 进行过研究。据我们所知，这是第一项研究 CMV-DNA 作为潜在病毒治疗靶点在非 GBM 婴儿脑肿瘤中的存在。

方法

使用实时聚合酶链反应 (PCR) 检查了 2006 年至 2016 年间接受手术的 36 名婴儿（年龄 < 24 个月）的非 GBM 脑肿瘤石蜡块的 CMV-DNA。CMV感染肺组织的石蜡块用作阳性对照。从各肿瘤组织中提取和扩增β2微球蛋白基因作为阳性内对照。我们还检测了 25 个脑膜膨出石蜡块的 CMV DNA 作为阴性组织对照。

结果

组织病理学诊断包括 13 个神经胶质/神经胶质瘤 (36.1%)、8 个室管膜瘤 (22.2%)、7 个髓母细胞瘤 (19.4%)、3 个脉络丛肿瘤 (8.3%)、2 个非典型畸胎样横纹肌瘤 (5.6%)、CN 胚胎肿瘤 (5.6%) 和 1 个生殖细胞肿瘤 (2.8%)。我们无法在所有检查的样本中检测到 CMV DNA。

结论

尽管 CMV 可能与 GBM 相关，但不能提出这种病毒在非 GBM 婴儿脑肿瘤发展中的作用。应该对更大系列的脑肿瘤进行进一步调查，以证实或排除我们的结论。