The cost of biodiesel production and the requirement of raw ingredients are the primary constraints that need to be addressed while searching for viable alternative fuels to petrol and diesel. Oleaginous yeasts are gaining wider acceptance as biofuel candidates among oil-rich crops/microbes. The present investigation aimed to integrate the agro-industrial wastewater stream as a nutrient source for the cultivation of oleaginous yeast and to use the resultant biomass and lipid as a feedstock for biofuel synthesis. The yeast biomass grown in sago processing wastewater contained 7.21% moisture content, 69.01% volatile matter, 12.61% fixed carbon, and 11.16% ash content. The ultimate analysis determined the contents of carbon (40.43%), nitrogen (5.14%), hydrogen (4.62%), sulfur (0.54%), and oxygen (49.27%). The heating value of yeast biomass was 16.54 MJ kg⁻¹. The thermal behavior of yeast biomass also suggests its potential use as an energy source. The FTIR spectrum of biomass had major lipid (3030–2800 cm⁻¹ and 1500–1350 cm⁻¹) and carbohydrate (1250 cm⁻¹ and 1000 cm⁻¹) functional peaks. Further FAME profiling revealed that the yeast biomass is primarily composed of stearic, oleic, linoleic, and linolenic acids, similar to the vegetable oils. The fuel characteristics of yeast biodiesel (SV, 168.87 mg KOH g⁻¹; IV, 120 mg I₂ 100 g⁻¹; CN, 61.79; and KV, 3.16 mm² s⁻¹) are also within the ASTM standard limits, suggesting that yeast biomass could be a sustainable and economically viable feedstock for both solid and liquid biofuel production.

在寻找可行的汽油和柴油替代燃料时，必须解决生物柴油生产的成本和原料要求。含油酵母作为富含燃料的农作物/微生物中的生物燃料候选物已得到越来越广泛的接受。本研究旨在整合农业工业废水流作为培养油质酵母的营养源，并将所得的生物质和脂质用作生物燃料合成的原料。在西米加工废水中生长的酵母生物量含有7.21％的水分，69.01％的挥发性物质，12.61％的固定碳和11.16％的灰分。最终分析确定了碳（40.43％），氮（5.14％），氢（4.62％），硫（0.54％）和氧（49.27％）的含量。酵母生物量的热值为16。^-1。酵母生物质的热行为也表明其潜在地用作能源。生物质的FTIR光谱具有主要的脂质（3030-2800 cm ^-1和1500-1350 cm ^-1）和碳水化合物（1250 cm ^-1和1000 cm ^-1）的功能峰。进一步的FAME分析表明，酵母生物量主要由硬脂酸，油酸，亚油酸和亚麻酸组成，类似于植物油。酵母生物柴油的燃料特性（SV，168.87 mg KOH g ^-1 ; IV，120 mg I ₂ 100 g ^-1 ; CN，61.79; KV，3.16 mm ² s ^-1）也在ASTM标准限制之内，这表明酵母生物质可能是固体和液体生物燃料生产的可持续且经济可行的原料。