Partially purified tannase of Talaromyces verruculosus (305.6 U/mL) was immobilized on calcium alginate beads (8.0 ± 1.0 mm) using 1% glutaraldehyde as a cross-linking agent. Immobilized enzyme preparation retained (39.4%) 120.4 U/mL tannase activity, 79.6% immobilization yield, and 49.5% efficiency. Immobilized preparation was characterized by SEM and it revealed the changed topology of the immobilized bead. Functional groups present in immobilized beads were examined by FTIR spectra. Immobilized tannase was found optimally active at pH 8.0 and 60 °C, whereas the stability was obtained between 30 and 60 °C, but at high (9.0) and low pH (4.0), immobilized tannase was also found very stable. Beads were found stable up to 9th cycle of reuse with 49.27% relative activity. After the treatment by immobilized tannase, 78.02 ± 1.93, 72.24 ± 2.05, 65.29 ± 1.21, 59.06 ± 0.96, and 64.61 ± 1.07% tannin reduction were measured in black tea product 1, black tea product 2, black tea product 3, green tea product 1, and green tea product 2, respectively. The greatest change in pH, turbidity, and antioxidant activity was found in black tea product 2 infusion while the least change was obtained in green tea product 2 infusion after the tannase treatment.

部分纯化的Talaromyces verruculosus鞣酸酶使用1％戊二醛作为交联剂，将（305.6 U / mL）（305.6 U / mL）固定在藻酸钙珠（8.0±1.0 mm）上。固定化酶制剂保留（39.4％）120.4 U / mL鞣酸酶活性，79.6％固定化产率和49.5％效率。固定化的制备物通过SEM进行表征，并且揭示了固定化的珠的改变的拓扑。通过FTIR光谱检查固定化珠中存在的官能团。发现固定的鞣酸酶在pH 8.0和60°C时具有最佳活性，而在30至60°C的温度下具有稳定性，但在高（9.0）和低pH（4.0）时，也发现固定的鞣酸酶非常稳定。发现珠粒在重复使用的第9个周期内稳定，相对活性为49.27％。固定化鞣酶处理后，分别为78.02±1.93、72.24±2.05、65.29±1.21、59.06±0.96和64.61±1。在红茶产品1，红茶产品2，红茶产品3，绿茶产品1和绿茶产品2中，单宁减少了07％。在鞣酸酶处理后，红茶产品2浸入液的pH，浊度和抗氧化活性变化最大，而绿茶产品2浸入液的变化最小。