The LC/MS-based exo-proteome analysis of thermophilic fungus Thermomyces lanuginosus showed 22.59% (40 proteins) of the total identified proteins (177) as CAZymes (carbohydrate-active enzymes). The CAZymes were primarily represented by glycosyl hydrolases (72.5%) belonging to 21 different GH families. Xylanase (GH11) was found to be the major protein (24.3%) in addition to the β-glucanase and another complex polysaccharide-degrading (carbohydrate-active enzymes) CAZymes in the secretome. FPLC-based fractionation of secretome was employed to identify proteins responsible for enhancing the catalytic efficacy of Cellic Ctec2 during hydrolysis of acid pre-treated lignocellulosics and eventually xylanase (GH11) and β-glucanases (GH64 and GH81) were identified and purified. The purified xylanase when supplemented with Cellic Ctec2 resulted in 2.05-, 1.79-, and 1.60-fold increase in the release of glucose from acid-treated bagasse at 10, 15, and 20% substrate loading rate, respectively when compared with the control. Similarly, enhanced hydrolysis of acid pre-treated corn residue and rice straw was observed upon supplementation with xylanase. Spiking benchmark cellulase with purified GH64 and GH81 also resulted in 1.18- and 1.23-fold enhanced hydrolysis of acid pre-treated sugarcane bagasse. The supplementation of xylanase (1000 units/g substrate) was found to reduce the Cellic Ctec2 loading rate from 36 mg/g substrate by 2.70-, 2.88-, and 2.57-fold required for hydrolysis of acid treated bagasse, corn residue, and rice straw, respectively, at high substrate loading rate (20%) and thus, it is an important candidate for economizing the 2G ethanol process.

基于LC / MS的嗜热真菌嗜热霉菌的蛋白质组分析在总鉴定蛋白质（177）中显示22.59％（40种蛋白质）为CAZymes（碳水化合物活性酶）。CAZymes主要由属于21个不同GH家族的糖基水解酶（72.5％）代表。发现木糖酶（GH11）是分泌蛋白组中除β-葡聚糖酶和另一种降解多糖的复合酶（碳水化合物活性酶）以外的主要蛋白质（24.3％）。使用基于FPLC的分泌组分离技术鉴定负责在酸性预处理木质纤维素水解过程中增强Cellic Ctec2催化功效的蛋白质，最终鉴定并纯化了木聚糖酶（GH11）和β-葡聚糖酶（GH64和GH81）。纯化的木聚糖酶补充Cellic Ctec2后，在10时从酸处理过的蔗渣中释放的葡萄糖增加了2.05、1.79和1.60倍。与对照相比，分别为15和20％的基板上样率。类似地，在补充木聚糖酶后，观察到酸预处理的玉米残留物和稻草的水解增强。掺入纯化的GH64和GH81的基准纤维素酶还导致酸预处理的甘蔗渣的水解度提高了1.18和1.23倍。发现补充木聚糖酶（1000单位/ g底物）可使酸处理的蔗渣，玉米残渣和大米水解所需的细胞Ctec2加载速率从36 mg / g底物降低2.70、2.88和2.57倍。秸秆分别在较高的底物负载率（20％）下运行，因此，它是节省2G乙醇工艺的重要候选人。补充木聚糖酶后，酸预处理的玉米残留物和稻草的水解作用增强。掺入纯化的GH64和GH81的基准纤维素酶还导致酸预处理的甘蔗渣的水解度提高了1.18和1.23倍。发现补充木聚糖酶（1000单位/ g底物）可使酸处理的蔗渣，玉米残渣和大米水解所需的细胞Ctec2加载速率从36 mg / g底物降低2.70、2.88和2.57倍。秸秆分别在较高的底物负载率（20％）下运行，因此，它是节省2G乙醇工艺的重要候选人。补充木聚糖酶后，酸预处理的玉米残留物和稻草的水解作用增强。掺入纯化的GH64和GH81的基准纤维素酶还导致酸预处理的甘蔗渣的水解度提高了1.18和1.23倍。发现补充木聚糖酶（1000单位/ g底物）可使酸处理的蔗渣，玉米残渣和大米水解所需的细胞Ctec2加载速率从36 mg / g底物降低2.70、2.88和2.57倍。秸秆分别在较高的底物负载率（20％）下运行，因此，它是节省2G乙醇工艺的重要候选人。酸预处理甘蔗渣的水解度提高了23倍。发现补充木聚糖酶（1000单位/ g底物）可使酸处理的蔗渣，玉米残渣和大米水解所需的细胞Ctec2加载速率从36 mg / g底物降低2.70、2.88和2.57倍。秸秆分别在较高的底物负载率（20％）下运行，因此，它是节省2G乙醇工艺的重要候选人。酸预处理甘蔗渣的水解度提高了23倍。发现补充木聚糖酶（1000单位/ g底物）可使酸处理的蔗渣，玉米残渣和大米水解所需的细胞Ctec2加载速率从36 mg / g底物降低2.70、2.88和2.57倍。秸秆分别在较高的底物负载率（20％）下运行，因此，它是节省2G乙醇工艺的重要候选人。