Although recent studies have explored dissimilatory nitrate reduction to ammonium (DNRA), little is known about the dependence of this process on temperature. A laboratory experiment was conducted to investigate if temperature regulates nitrate (NO₃ˉ) reduction by DNRA and denitrification. Soil samples were incubated at 10 to 40 °C for 28 days, and ¹⁵N tracer methods were applied to estimate the reduction of ¹⁵NO₃ˉ to ¹⁵NH₄⁺ by DNRA organisms and to ¹⁵N₂O or ¹⁵N₂ by denitrifiers. Raising the temperature from 10 to 40 °C increased DNRA, which contributed to enrichment of the NH₄⁺ pool, particularly at 40 °C. The percentage of ¹⁵NO₃ˉ reduced by DNRA was from 4 to 30% of that reduced by denitrification, and decreased with increasing temperature. DNRA and denitrification were positively correlated with the abundance of nrfA and nosZ genes, respectively. The accumulation of ¹⁵NH₄⁺ slowed in the final 12 h of sampling times, which may be due to a re-conversion of ¹⁵NH₄⁺ to ¹⁵NO₃ˉ by nitrification. High temperatures (30 to 40 °C) enhanced ¹⁵N₂ production, which suggests that temperature plays an important role in regulating complete denitrification from NO₃ˉ to N₂.

尽管最近的研究探索了异化硝酸盐还原为铵盐（DNRA），但对于该过程对温度的依赖性知之甚少。一个实验室实验以研究是否硝酸盐温度时调节（NO ₃ ˉ）由DNRA和反硝化作用的降低。土壤样品在10〜40℃下培养28天，^15种施加Ñ示踪方法来估计的减少¹⁵ NO ₃ ˉ至¹⁵ NH ₄ ⁺通过DNRA生物体和¹⁵ Ñ ₂ O或¹⁵ Ñ ₂通过反硝化器。将温度从10升高到40°C会增加DNRA，这有助于NH ₄ ⁺库的富集，尤其是在40°C时。DNRA还原的¹⁵ NO ₃的百分比为反硝化还原的15％至4％至30％，并且随着温度的升高而降低。DNRA和反硝化分别与nrfA和nosZ基因的丰度正相关。在最后的12小时采样时间内，¹⁵ NH ₄ ⁺的积累变慢，这可能是由于¹⁵ NH ₄ ⁺转化为¹⁵ NO ₃所致ˉ经硝化作用。高的温度（30〜40℃）提高¹⁵ Ñ ₂生产，这表明温度对从NO调节完成脱氮重要作用₃ ˉ到N ₂。