In this study, multiple and rapid propagations of Staurogyne repens (Nees) Kuntze with tissue culture techniques were aimed. The nodal explants of S. repens were cultured for multiple shoot regeneration in culture medium supplemented with different rates of 6-Benzilaminopurin (BAP) and Kinetin (KIN) alone (0.25–1.50 mg/L) or combinations with 0.25 mg/L Indole-3-acetic acid (IAA). Generally, shoot regeneration capabilities of the explants were high due to the growth regulators used. Shoot regeneration frequencies were ranked between 77.77% and 100.00% in BAP-IAA and KIN-IAA applications. In BAP-IAA application, the average number of shoots was recorded between 2.53–16.11 shoots/explant and the highest number of shoots per explant (16.11 shoots/explant) was obtained in culture medium containing 1.25 mg/L BAP. As the BAP level used increased, the number of regenerated shoots increased. In KIN-IAA application, mean shoot counts were recorded as 2.66–10.27 shoots/explant, and maximum shoots per explant (10.27 shoots/explant) was determined in culture medium containing 0.50 mg/L KIN. When shoot lengths were examined, longer shoots were seen in KIN-IAA application compared to BAP-IAA application. The longest shoot was obtained as 2.82 cm in the culture medium fortified with 1.50 mg/L KIN. Regenerated shoots for in vitro rooting were transferred to the growth culture containing different concentrations of IAA and 1-Naphthaleneacetic acid (NAA). The best values in terms of number and length of roots per shoot were determined in a growth culture containing 0.25 mg/L IAA. The rooted shoots were accustomed to external conditions successfully.

在这项研究中，目标是利用组织培养技术快速繁殖白头翁（Nees）Kuntze。的节外植体S.三叶在补充有不同比例的6-苄基氨基嘌呤（BAP）和激动素（KIN）（0.25–1.50 mg / L）或0.25 mg / L吲哚-3-乙酸（IAA）的组合培养基中培养多次芽再生。通常，由于使用了生长调节剂，外植体的芽再生能力很高。在BAP-IAA和KIN-IAA应用中，嫩芽再生频率介于77.77％和100.00％之间。在BAP-IAA应用中，记录的平均芽数为2.53–16.11芽/每株，在含有1.25 mg / L BAP的培养基中，每株芽的最高芽数（16.11芽/每株）。随着使用的BAP水平增加，再生芽的数量增加。在KIN-IAA应用中，平均芽数记录为2.66–10.27芽/植株，在含有0.50 mg / L KIN的培养基中确定每个外植体的最大芽数（10.27个芽/外植体）。当检查芽长时，与BAP-IAA施用相比，在KIN-IAA施用中观察到更长的芽。在以1.50 mg / L KIN强化的培养基中，最长的芽长为2.82 cm。将用于再生生根的再生芽转移至含有不同浓度的IAA和1-萘乙酸（NAA）的生长培养物中。在含有0.25 mg / L IAA的生长培养物中确定了每枝的根数和根长方面的最佳值。生根的芽成功地适应了外部条件。与BAP-IAA应用相比，KIN-IAA应用中观察到更长的芽。在以1.50 mg / L KIN强化的培养基中，最长的芽长为2.82 cm。将用于再生生根的再生芽转移至含有不同浓度的IAA和1-萘乙酸（NAA）的生长培养物中。在含有0.25 mg / L IAA的生长培养物中确定了每枝的根数和根长方面的最佳值。生根的芽成功地适应了外部条件。与BAP-IAA应用相比，KIN-IAA应用中观察到更长的芽。在以1.50 mg / L KIN强化的培养基中，最长的芽长为2.82 cm。将用于再生生根的再生芽转移至含有不同浓度的IAA和1-萘乙酸（NAA）的生长培养物中。在含有0.25 mg / L IAA的生长培养物中确定了每枝的根数和根长方面的最佳值。生根的芽成功地适应了外部条件。在含有0.25 mg / L IAA的生长培养物中确定了每枝的根数和根长方面的最佳值。生根的芽成功地适应了外部条件。在含有0.25 mg / L IAA的生长培养物中确定了每枝的根数和根长方面的最佳值。生根的芽成功地适应了外部条件。