The translation initiation factor 4E (eIF4E) has been implicated in naturally-occurring resistance to the Potato Virus Y (PVY) determined by the pot-1 locus in tomato. The susceptible potato varieties Russet Norkotah, Silverton Russet, and Classic Russet, along with the Michigan State University breeding line MSE149-5Y, were transformed with the pot-1 gene from Solanum hirsutum to achieve PVY resistance. Transgenic potato events and their non-transgenic controls were screened for PVY resistance against three PVY strains (PVY^O, PVY^N:O, PVY^NTN) by enzyme-linked immunosorbent assay (ELISA) in greenhouse and in field trials. The event A95.87.05 (Classic Russet) and MSE149.87.22 (MSE149-5Y) had resistance against all three PVY isolates tested in both foliage and tuber under either greenhouse or field conditions. In contrast, SR.87.62 (Silverton Russet) and RN1.87.09 (Russet Norkotah), displayed moderate susceptibility to PVY in foliage during the growing season and increased level of infection in tubers. Transformation of PVY-susceptible potato varieties with the tomato pot-1 gene can be a useful tool for improving PVY resistance.

翻译起始因子4E（eIF4E）与番茄pot-1基因座确定的对马铃薯病毒Y（PVY）的天然抗性有关。将易感马铃薯品种Russet Norkotah，Silverton Russet和Classic Russet以及密歇根州立大学的育种系MSE149-5Y，用来自茄子的pot-1基因进行转化，以获得PVY抗性。筛选转基因马铃薯事件及其非转基因对照对三种PVY菌株（PVY ^O，PVY ^N：O，PVY ^NTN通过酶联免疫吸附测定（ELISA）在温室和田间试验中进行。事件A95.87.05（经典Russet）和MSE149.87.22（MSE149-5Y）对在温室或田间条件下在叶子和块茎中测试的所有三种PVY分离株均具有抗性。相反，SR.87.62（Silverton Russet）和RN1.87.09（Russet Norkotah）在生长季节对叶片中的PVY表现出中等敏感性，并且块茎的感染水平增加。番茄pot-1基因对PVY易感马铃薯品种的转化可能是提高PVY抗性的有用工具。