Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics. Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity. Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified. Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.

中文翻译：

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伊朗北部靠近里海的人类包虫病的系统地理学和遗传多样性，重点关注细粒棘球绦虫 Sensu Stricto 复合体

背景：人类棘球蚴病是由细粒棘球绦虫复合体的绦虫引起的环状人畜共患感染。对棘球蚴属线粒体基因组数据的检测可以反映其分类状况、遗传多样性和种群结构遗传学。方法： 1995 年 3 月至 2018 年 5 月，从伊朗马赞达兰省采集组织学证实为 CE 患者的 52 份福尔马林固定石蜡包埋（FFPE）组织样本。通过针对细胞色素 c 氧化酶亚基 1 (cox1) 基因的聚合酶链反应法进行扩增。所有 PCR 扩增子都进行了系统发育分析和遗传多样性测序。结果：分子分析表明，50株（96.1%）和2株（3.84%）株分别为G1和G3大肠杆菌。granulosus 基因型，分别。DNA 序列分析表明 G1（单倍型多样性：0.830）和 G3 基因型（Hd：1.00）的基因多样性很高。基于多序列比对分析，明确鉴定出 7 个（13.46%；G1 基因型）和 2 个（3.84%；G3 基因型）新的单倍型。结论： G3基因型（Buffalo株）从该地区的两个人包虫病分离株中鉴定出来。目前的研究加强了我们对伊朗北部临床 CE 分离株中棘球蚴寄生虫对人类的分类状态、传播模式以及该寄生虫的异质性方面的了解。G3 基因型）新的单倍型被明确鉴定。结论： G3基因型（Buffalo株）从该地区的两个人包虫病分离株中鉴定出来。目前的研究加强了我们对伊朗北部临床 CE 分离株中棘球蚴寄生虫对人类的分类状态、传播模式以及该寄生虫的异质性方面的了解。G3 基因型）新的单倍型被明确鉴定。结论： G3基因型（Buffalo株）从该地区的两个人包虫病分离株中鉴定出来。目前的研究加强了我们对伊朗北部临床 CE 分离株中棘球蚴寄生虫对人类的分类状态、传播模式以及该寄生虫的异质性方面的了解。