An action of the IR laser pulses with a wavelength of 1.56 μm, moderate intensity, and various durations on a collagen framework structure and sclera tissue permeability is considered. It is shown that the temperature thresholds of collagen denaturation in tissues under the laser irradiation are substantially lower as compared to those under conventional homogeneous heating in a calorimeter oven. A tissue matrix modified at temperatures below the denaturation threshold is observed in images obtained by nonlinear optical microscopy in the regime of second harmonic generation. The modification has specific features under long-duration (with a duration longer than 1 s) and short-duration (10 – 30 ms) action: separation of a fibre bundle into groups with distorted passage in the first case and bundle splitting to chaotically directed fibres in the second case. The character of the tissue change determines the coefficient of organic substance diffusion into a tissue material. After matrix amorphisation (collagen denaturation) or distortion of the fibre group arrangement in the collagen bundle, the diffusion coefficient falls by 10 % – 40 %, whereas after bundle splitting it increases by 25 % – 50 % as compared to the value of (3 0.1) 10⁻⁶ cm s⁻¹, which is specific for intact tissue. The results obtained make us to assume that the specific disordering of the tissue collagen framework under the short-duration action may be used for increasing the permeability of the scleral tissue.

考虑具有1.56μm的波长，中等强度和各种持续时间的IR激光脉冲对胶原框架结构和巩膜组织渗透性的作用。结果表明，与在热量计烘箱中常规均匀加热的情况相比，激光辐照下的组织中胶原蛋白变性的温度阈值要低得多。在二次谐波产生范围内，通过非线性光学显微镜获得的图像中观察到在低于变性阈值的温度下改性的组织基质。该修改在长期（持续时间大于1 s）和短期（10 – 30 ms）操作下具有特定功能：在第一种情况下，将纤维束分成几组并通过扭曲的通道，在第二种情况下，将纤维束分裂成混乱的定向纤维。组织变化的特性决定了有机物质扩散到组织材料中的系数。胶原蛋白束中的基质无定形（胶原蛋白变性）或纤维基团排列变形后，扩散系数下降10％– 40％，而束分裂后，扩散系数与（3）值相比增加25％– 50％ 0.1）10^-6 cm s ^-1，对于完整的组织是特定的。获得的结果使我们假设，在短期作用下组织胶原蛋白骨架的特定紊乱可用于增加巩膜组织的通透性。