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Genome-wide identification and characterization of COMT gene family during the development of blueberry fruit
BMC Plant Biology ( IF 4.3 ) Pub Date : 2021-01-06 , DOI: 10.1186/s12870-020-02767-9
Yushan Liu 1, 2 , Yizhou Wang 3, 4 , Jiabo Pei 1, 2, 5 , Yadong Li 1 , Haiyue Sun 1
Affiliation  

Caffeic acid O-methyltransferases (COMTs) play an important role in the diversification of natural products, especially in the phenylalanine metabolic pathway of plant. The content of COMT genes in blueberry and relationship between their expression patterns and the lignin content during fruit development have not clearly investigated by now. Ninety-two VcCOMTs were identified in Vaccinium corymbosum. According to phylogenetic analyses, the 92 VcCOMTs were divided into 2 groups. The gene structure and conserved motifs within groups were similar which supported the reliability of the phylogenetic structure groupings. Dispersed duplication (DSD) and whole-genome duplication (WGD) were determined to be the major forces in VcCOMTs evolution. The results showed that the results of qRT-PCR and lignin content for 22 VcCOMTs, VcCOMT40 and VcCOMT92 were related to lignin content at different stages of fruit development of blueberry. We identified COMT gene family in blueberry, and performed comparative analyses of the phylogenetic relationships in the 15 species of land plant, and gene duplication patterns of COMT genes in 5 of the 15 species. We found 2 VcCOMTs were highly expressed and their relative contents were similar to the variation trend of lignin content during the development of blueberry fruit. These results provide a clue for further study on the roles of VcCOMTs in the development of blueberry fruit and could promisingly be foundations for breeding blueberry clutivals with higher fruit firmness and longer shelf life.

中文翻译:

蓝莓果实发育过程中COMT基因家族的全基因组鉴定与表征

咖啡酸O-甲基转移酶(COMTs)在天然产物的多样化中发挥着重要作用,特别是在植物的苯丙氨酸代谢途径中。蓝莓中COMT基因的含量及其表达模式与果实发育过程中木质素含量的关系目前尚未明确。在 Vaccinium corymbosum 中鉴定了 92 个 VcCOMT。根据系统发育分析,92 个 VcCOMT 分为 2 组。组内的基因结构和保守基序相似,支持系统发育结构分组的可靠性。分散重复 (DSD) 和全基因组重复 (WGD) 被确定为 VcCOMT 进化的主要力量。结果表明,22 个 VcCOMT 的 qRT-PCR 结果和木质素含量,VcCOMT40和VcCOMT92与蓝莓果实发育不同阶段木质素含量有关。我们鉴定了蓝莓中的COMT基因家族,并对15种陆生植物的系统发育关系和15种中5种COMT基因的基因重复模式进行了比较分析。我们发现2个VcCOMTs高表达,其相对含量与蓝莓果实发育过程中木质素含量的变化趋势相似。这些结果为进一步研究 VcCOMTs 在蓝莓果实发育中的作用提供了线索,并有望成为培育具有更高果实硬度和更长货架期的蓝莓品种的基础。并对15种陆生植物的系统发育关系和15种植物中5种的COMT基因重复模式进行了比较分析。我们发现2个VcCOMTs高表达,其相对含量与蓝莓果实发育过程中木质素含量的变化趋势相似。这些结果为进一步研究 VcCOMTs 在蓝莓果实发育中的作用提供了线索,并有望成为培育具有更高果实硬度和更长货架期的蓝莓品种的基础。并对15种陆生植物的系统发育关系和15种植物中5种的COMT基因重复模式进行了比较分析。我们发现2个VcCOMTs高表达,其相对含量与蓝莓果实发育过程中木质素含量的变化趋势相似。这些结果为进一步研究 VcCOMTs 在蓝莓果实发育中的作用提供了线索,并有望成为培育具有更高果实硬度和更长货架期的蓝莓品种的基础。我们发现2个VcCOMTs高表达,其相对含量与蓝莓果实发育过程中木质素含量的变化趋势相似。这些结果为进一步研究 VcCOMTs 在蓝莓果实发育中的作用提供了线索,并有望成为培育具有更高果实硬度和更长货架期的蓝莓品种的基础。我们发现2个VcCOMTs高表达,其相对含量与蓝莓果实发育过程中木质素含量的变化趋势相似。这些结果为进一步研究 VcCOMTs 在蓝莓果实发育中的作用提供了线索,并有望成为培育具有更高果实硬度和更长货架期的蓝莓品种的基础。
更新日期:2021-01-06
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