Phosphate is the major ingredient of bone tissue, and is also an important component of commercial bone substitute materials, bone scaffolds, and implant surface coatings. With the dissolution of the bone substitute materials and the degradation by cells, local ion concentrations will change and affect bone tissue reconstruction. Bone marrow -derived mesenchymal stem cells (BM-MSCs) are main autologous cells to repair injured bone. When bone injure occurs, BM-MSCs migrate to the damaged area, differentiate into osteoblasts, and secrete bioactive factors to promote bone tissue repaired. This study aimed to investigate the effect of inorganic phosphate (Pi) at a series of concentration on migration and osteogenic differentiation of human bone marrow -derived mesenchymal stem cells(hBM-MSCs). The culture of hBM-MSCs in mediums with different concentration of Pi from 2 mM to 10 mM were performed. HBM-MSCs migration were examined with transwell assays. HBM-MSCs proliferation were evaluated by cell counting kit-8 colorimetric method. Osteogenic genes expression were analyzed by real-time reverse transcriptase polymerase chain reaction. Mineralized nodules formation were demonstrated by Alizarin red staining. 4–10 mM Pi could effectively promote the migration of hBM-MSCs at 12 h and 18 h. There was no significant difference in the migration number of hBM-MSCs in Pi culture mediums at a concentration of 6, 8, and10mM. 2–10 mM Pi could promote the proliferation of hBM-MSCs to varying degrees in the observation period, while 4–10 mM Pi could promote the osteogenic differentiation and mineralization of hBM-MSCs. The findings in our study showed 4-10 mM Pi could promote the migration, osteogenic differentiation, and mineralization of hBM-MSCs.

中文翻译：

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无机磷酸盐对骨髓间充质干细胞迁移和成骨分化的影响

磷酸盐是骨​​组织的主要成分，也是商业骨替代材料、骨支架和植入物表面涂层的重要成分。随着骨替代材料的溶解和细胞的降解，局部离子浓度会发生变化，影响骨组织重建。骨髓间充质干细胞（BM-MSCs）是修复受损骨的主要自体细胞。当骨损伤发生时，BM-MSCs迁移至损伤部位，分化为成骨细胞，并分泌生物活性因子促进骨组织修复。本研究旨在探讨一系列浓度的无机磷酸盐(Pi)对人骨髓间充质干细胞(hBM-MSCs)迁移和成骨分化的影响。在具有从 2 mM 到 10 mM 的不同 Pi 浓度的培养基中进行 hBM-MSCs 的培养。用 transwell 测定法检查 HBM-MSCs 迁移。采用细胞计数 kit-8 比色法评估 HBM-MSCs 增殖情况。通过实时逆转录酶聚合酶链反应分析成骨基因的表达。茜素红染色证实了矿化结节的形成。4-10 mM Pi 可有效促进 hBM-MSCs 在 12 h 和 18 h 的迁移。在浓度为6、8和10mM的Pi培养基中hBM-MSCs的迁移数没有显着差异。在观察期内，2~10 mM Pi可以不同程度地促进hBM-MSCs的增殖，而4~10 mM Pi可以促进hBM-MSCs的成骨分化和矿化。