Urinary retention (UR) is a common urinary system disease can be caused by urinary tract obstruction with numerous reasons, however, the role of urine microbes in these disorders is still poorly understood. The aim of this study was to identify the urine microbial features of two common types of obstructive UR, caused by urinary stones or urinary tract tumors, with comparison to healthy controls. Urine samples were collected from a cohort of 32 individuals with stone UR, 25 subjects with tumor UR and 25 healthy controls. The urine microbiome of all samples was analyzed using high-throughput 16S rRNA (16S ribosomal RNA) gene sequencing. We observed dramatically increased urine microbial richness and diversity in both obstructive UR groups compared to healthy controls. Despite different origins of UR, bacteria such as Pseudomonas, Acinetobacter and Sphingomonas were enriched, while Lactobacillus, Streptococcus, Gardnerella, Prevotella and Atopobium were decreased in both UR groups in comparison with healthy controls, exhibited an approximate urine microbial community and functional characteristics of two types of obstructive UR. Furthermore, disease classifiers were constructed using specific enriched genera in UR, which can distinguish stone UR or tumor UR patients from healthy controls with an accuracy of 92.29% and 97.96%, respectively. We presented comprehensive microbial landscapes of two common types of obstructive urinary retention and demonstrated that urine microbial features of these patients are significantly different from that of healthy people. The urine microbial signatures would shed light on the pathogenesis of these types of urinary retention and might be used as potential classification tools in the future.

中文翻译：

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新一代测序揭示梗阻性尿潴留患者尿液微生物群失调


尿潴留（UR）是一种常见的泌尿系统疾病，可由多种原因引起的尿路梗阻引起，然而，尿液微生物在这些疾病中的作用仍知之甚少。本研究的目的是与健康对照者进行比较，以确定由尿路结石或尿路肿瘤引起的两种常见类型的梗阻性 UR 的尿液微生物特征。尿液样本采集自 32 名结石性 UR 患者、25 名肿瘤性 UR 受试者和 25 名健康对照者。使用高通量 16S rRNA（16S 核糖体 RNA）基因测序分析所有样本的尿液微生物组。我们观察到，与健康对照组相比，两个阻塞性 UR 组的尿液微生物丰富度和多样性显着增加。尽管UR的来源不同，但与健康对照相比，两个UR组的细菌如假单胞菌、不动杆菌和鞘氨醇单胞菌均富集，而乳杆菌、链球菌、加德纳菌、普雷沃氏菌和Atopobium均减少，表现出与健康对照相似的尿液微生物群落和功能特征。阻塞性 UR 的类型。此外，利用 UR 中特定的富集属构建了疾病分类器，可以将结石 UR 或肿瘤 UR 患者与健康对照区分开来，准确率分别为 92.29% 和 97.96%。我们提出了两种常见类型的梗阻性尿潴留的全面微生物景观，并证明这些患者的尿液微生物特征与健康人显着不同。尿液微生物特征将揭示这些类型的尿潴留的发病机制，并可能在未来用作潜在的分类工具。