Biological information can be encoded in the dynamics of signaling components which has been implicated in a broad range of physiological processes including stress response, oncogenesis, and stem cell differentiation. To study the complexity of information transfer across the eukaryotic promoter, we screened 119 dynamic conditions-modulating the frequency, intensity, and pulse width of light-regulating the binding of an epigenome editor to a fluorescent reporter. This system revealed highly tunable gene expression and filtering behaviors and provided the most comprehensive quantification to date of the maximum amount of information that can be reliably transferred across a promoter as ~1.7 bits. Using a library of over 100 orthogonal epigenome editors, we further determined that chromatin state could be used to tune mutual information and expression levels, as well as completely alter the input-output transfer function of the promoter. This system unlocks the information-rich content of eukaryotic epigenome editing.

中文翻译：

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映射表观基因组编辑的动态传递函数

可以在信号传导动力学中编码生物信息，这些信号涉及广泛的生理过程，包括应激反应，肿瘤发生和干细胞分化。为了研究跨真核启动子的信息传递的复杂性，我们筛选了119个动态条件，这些条件调节光的频率，强度和脉冲宽度，从而调节表观基因组编辑器与荧光报告分子的结合。该系统揭示了高度可调的基因表达和过滤行为，并提供了迄今为止最全面的量化信息，该信息可以可靠地在启动子上以约1.7位的形式传递最大信息。使用超过100个正交表观基因组编辑器的库，我们进一步确定染色质状态可用于调节相互信息和表达水平，以及完全改变启动子的输入-输出传递函数。该系统解锁了真核表观基因组编辑的丰富信息内容。