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Fluorescent bacterial biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz radiation
Biomedical Optics Express ( IF 2.9 ) Pub Date : 2021-01-05 , DOI: 10.1364/boe.412074
Danil S. Serdyukov , Tatiana N. Goryachkovskaya , Irina A. Mescheryakova , Sergei A. Kuznetsov , Vasiliy M. Popik , Sergey E. Peltek

A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.

中文翻译:

对太赫兹辐射敏感的荧光细菌生物传感器大肠杆菌/ pTdcR-TurboYFP

通过用质粒转化大肠杆菌E. coli)细胞,开发了一种对太赫兹(THz)辐射敏感的荧光生物传感器E. coli / pTdcR-TurboYFP ,其中tdcR基因的启动子控制黄色荧光蛋白TurboYFP的表达。生物传感器在各种容器和营养培养基中暴露于太赫兹辐射。发现荧光的阈值和动力学取决于照射条件。热激或化学应力导致没有荧光诱导。该生物传感器可用于研究THz辐射对tdcR启动子活性的影响,tdcR启动子参与苏氨酸和丝氨酸的转运和代谢。大肠杆菌
更新日期:2021-02-01
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