Abstract

Female fertility depends greatly on the capacity of the uterus to recognize and eliminate microbial infections, a major reason of inflammation in the endometrium in many species. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression and alternative splicing in the porcine endometrium in the mid-luteal phase of the estrous cycle during LPS-stimulated inflammation using RNA-seq technology. The endometrial slices were incubated in vitro in the presence of LPS and PPARγ agonists—PGJ₂ or pioglitazone and antagonist—T0070907. We identified 222, 3, 4, and 62 differentially expressed genes after LPS, PGJ₂, pioglitazone, or T0070907 treatment, respectively. In addition, we detected differentially alternative spliced events: after treatment with LPS-78, PGJ₂-60, pioglitazone-52, or T0070907-134. These results should become a basis for further studies explaining the mechanism of PPARγ action in the reproductive system in pigs.

中文翻译：

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LPS 诱导炎症后猪子宫内膜的转录组分析：PPAR-γ 配体的体外作用†

摘要

女性的生育能力在很大程度上取决于子宫识别和消除微生物感染的能力，这是许多物种子宫内膜炎症的主要原因。本研究旨在确定过氧化物酶体增殖物激活受体 γ (PPARγ) 配体对 LPS 刺激炎症期间 LPS 刺激炎症过程中发情周期黄体中期猪子宫内膜转录组基因表达和可变剪接的体外影响。测序技术。_{子宫内膜切片在 LPS 和 PPARγ 激动剂 - PGJ 2}或吡格列酮和拮抗剂 - T0070907存在下体外孵育。我们在 LPS、PGJ _{2后鉴定了 222、3、4 和 62 个差异表达基因}、吡格列酮或 T0070907 治疗。此外，我们检测到不同的可变剪接事件：LPS-78、PGJ ₂ -60、吡格列酮-52 或 T0070907-134 治疗后。这些结果应成为进一步研究解释PPARγ在猪生殖系统中作用机制的基础。