ABSTRACT

Fluopyram, flupyradifurone, and indaziflam are novel systemic pesticides developed by Bayer. Based on available data, these pesticides appears to have a favorable safety profile in the case of pesticide residue problems.

Herein, we report an effective analytical method for the determination of novel pesticides were developed using ultra-high performance liquid chromatography with tandem mass spectrometry coupled with a quick, easy, cheap, effective, rugged, and safe procedure. These target compounds were extracted with acetonitrile and cleaned up with PSA, and were separated successfully between 10.2 and 18.3 min using a C₁₈ chromatographic column connected to an electrospray ionization source. All the matrix-matched samples at three fortified levels (0.01, 0.1, and 0.5 mg kg⁻¹) provided satisfactory recoveries in the range of 80.6–93.5% with relative standard deviations below 10.8%. The limits of quantitation were 0.0055 mg kg⁻¹ with a limit of detection of 0.0015 mg kg⁻¹ for fluopyram in pistachio matrix, 0.0062 mg kg⁻¹ with a limit of detection of 0.0019 mg kg⁻¹ for flupyradifurone in date fruits and 0.0083 mg kg⁻¹ with a limit of detection of 0.0025 mg kg⁻¹ for indaziflam in soil complex matrix. The limit of quantification of studied pesticides was far below the maximum residue limit in the national MRLs so the proposed method is of great significance for establishing maximum residue limits in Iran. Date fruit real samples had not any flupyradifurone residue, and some pistachio and soil samples had trace fluopyram and indaziflam residues but there were below LOQs.

摘要

Fluopyram，flupyradifurone和indaziflam是拜耳开发的新型系统性农药。根据现有数据，在农药残留问题的情况下，这些农药似乎具有良好的安全性。

在这里，我们报告了一种有效的分析方法，用于测定新型农药，该方法是使用串联质谱联用的超高效液相色谱法结合快速，简便，廉价，有效，坚固和安全的程序开发的。这些目标化合物用乙腈萃取并用PSA净化，并使用连接至电喷雾电离源的C ₁₈色谱柱在10.2至18.3分钟之间成功分离。在三个强化水平（0.01、0.1和0.5 mg kg ^-1）下，所有与基质匹配的样品都能在80.6–93.5％的范围内提供令人满意的回收率，相对标准偏差低于10.8％。定量限为0.0055 mg kg ^-1，检出限为0.0015 mg kg^-1用于氟吡菌酰胺在开心果矩阵，0.0062毫克千克^-1与检测0.0019毫克公斤的限制^-1在日期水果和0.0083毫克公斤flupyradifurone ^-1与检测0.0025毫克公斤的限制^-1用于土壤indaziflam复数矩阵。研究农药的定量限远低于国家最大残留限量中的最大残留限，因此，所提出的方法对于确定伊朗的最大残留限具有重要意义。枣果实样中没有氟吡呋喃酮残留，一些开心果和土壤样本中有微量的氟吡喃和茚达那非残留，但最低定量限以下。