ABSTRACT

Electrical signals in plants caused by external stimuli are capable of inducing various physiological responses. The mechanisms of transformation of a long-distance electrical signal (ES) into a functional response remain largely unexplored and require additional research. In this work, we investigated the role of calcium ions in the development of ES-induced respiratory response. Gradual heating of the leaf causes the propagation of variation potential (VP) in the pea seedling. The propagation of VP leads to a transient activation of respiration in an unaffected leaf. During the VP generation, a transient increase in the intracellular calcium concentration takes place. A calcium channel blocker inhibits the respiratory response, and a calcium ionophore induces the activation of respiration. Inhibitory analysis has showed that the VP-induced increase in respiration activity is probably associated with calcium-mediated activation of rotenone-insensitive alternative NADPH dehydrogenases in mitochondria.

摘要

植物中由外部刺激引起的电信号能够诱导各种生理反应。远距离电信号（ES）转换为功能响应的机制仍未开发，需要进一步研究。在这项工作中，我们调查了钙离子在ES诱发的呼吸反应中的作用。逐渐加热叶片会导致豌豆幼苗中变异电位（VP）的传播。VP的传播导致未受影响的叶片中呼吸的瞬时激活。在VP生成过程中，细胞内钙浓度会瞬时增加。钙通道阻滞剂抑制呼吸反应，钙离子载体诱导呼吸的激活。