ABSTRACT

Sageretia thea fruits (STF) are known to have a higher content of functional ingredients and nutrients than blueberries, but studies related to its pharmacological activity are insufficient. Thus, in this study, we investigated whether STF exhibits immune-enhancing activity through macrophage activation. STF increased the production of immunomodulators, cell viability and phagocytosis in RAW264.7 cells. The inhibition of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) blocked STF-mediated production of immunomodulators in RAW264.7 cells. Inhibition of mitogen-activated protein kinases (MAPKs), nuclear factor-kappaB (NF-κB) and phosphoinositide-3-kinase (PI3K)/protein kinase B (AKT) signalling pathway reduced STF-mediated production of immunomodulators and the activation of MAPK, NF-κB and PI3K/AKT signalling pathway by STF suppressed the inhibition of TLR2/TLR4. However, STF blocked the overproduction of immunomodulators in LPS-stimulated RAW264.7 cells. Taken together, STF induced macrophage activation through TLR2/TLR4-dependent activation of MAPK, NF-κB and PI3K/AKT signalling.

摘要

鼠尾草众所周知，水果（STF）的功能成分和营养成分含量高于蓝莓，但有关其药理活性的研究不足。因此，在这项研究中，我们调查了STF是否通过巨噬细胞激活表现出免疫增强活性。STF增加了RAW264.7细胞中免疫调节剂的产生，细胞活力和吞噬作用。Toll样受体2（TLR2）和Toll样受体4（TLR4）的抑制作用可阻断RAW264.7细胞中STF介导的免疫调节剂的产生。抑制有丝分裂原激活的蛋白激酶（MAPKs），核因子-κB（NF-κB）和磷酸肌醇-3-激酶（PI3K）/蛋白激酶B（AKT）信号通路减少了STF介导的免疫调节剂的产生和MAPK的激活，STF的NF-κB和PI3K / AKT信号通路抑制了TLR2 / TLR4的抑制。但是，STF阻止了LPS刺激的RAW264.7细胞中免疫调节剂的过度生产。总之，STF通过MAPK，NF-κB和PI3K / AKT信号的TLR2 / TLR4依赖性激活来诱导巨噬细胞激活。