Abstract This study investigated the effects of biofilm removal from the tooth-restoration surface on secondary caries lesion progression. Biofilms were grown for up to 28 days on resin-restored enamel-dentin disks with an interfacial gap with DMM or DMM + 1% sucrose under five different protocols of Visible Biofilm Removal (V-BR; n = 7): (1) without V-BR, (2) with partial V-BR, (3) with total V-BR, (4) with total V-BR and saliva reinoculation, and (5) without V-BR under 9 h of cariogenic challenge. V-BR was performed at 7, 14 and 21 days. ΔS and CFU counts served as outcome variables. Linear regression models showed that ΔS values were higher for outer lesions than wall lesions, and outer lesion progression generally increased over time (p < 0.01). All protocols tested, except total V-BR, increased the lactobacilli count (p < 0.005). V-BR did not influence the progression of caries lesions on the cavity wall in this biofilm model.

中文翻译：

---

从牙齿修复界面去除生物膜对继发龋病进展的影响：初步体外模型研究

摘要 本研究调查了从牙齿修复表面去除生物膜对继发性龋病病变进展的影响。在五种不同的可见生物膜去除方案（V-BR；n = 7）下，生物膜在与 DMM 或 DMM + 1% 蔗糖有界面间隙的树脂修复釉质牙本质盘上生长长达 28 天：(1) V-BR，(2) 部分 V-BR，(3) 总 V-BR，(4) 总 V-BR 和唾液再接种，以及 (5) 在 9 小时致龋挑战下没有 V-BR。V-BR 在第 7、14 和 21 天进行。ΔS 和 CFU 计数作为结果变量。线性回归模型显示，外部病变的 ΔS 值高于壁病变，并且外部病变进展通常随着时间的推移而增加（p < 0.01）。除了总 V-BR 外，所有测试的方案都增加了乳酸杆菌计数 (p < 0.005)。