An electroporation that formed the pores on cell membrane after application of high electric field pulses has been used the transformation and the sterilization of cell. In this paper, we reported the extraction method of nucleic acid in Escherichia coli and Saccharomyces cerevisiae using high electric field pulse application. The sample was 1.0 × 10–3 mol/L NaCl aqueous solution contained E. coli and S. cerevisiae. High voltage pulse is applied to the sample at room temperature. These results were shown as the following: (1) When a pulsed electric field of 4 ∼ 13 kV/mm is applied to E. coli and S. cerevisiae, the estimated amount of nucleic acid increases with the increases of strength of applied pulsed electric field. (2) In the case of E. coli, the 2–3 kbp band was confirmed at an electric field of 5 kV/mm or more, and the 10 kbp band was confirmed at an electric field of 8 kV/mm or more. (3) In the case of S. cerevisiae, the 2–3 kbp band was confirmed at an electric field of 9 kV/mm or more. the 10 kbp band could not be confirmed at 13 kV/mm or less.

中文翻译：

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使用高电场脉冲施加提取大肠杆菌和啤酒酵母中的核酸

在施加高电场脉冲后在细胞膜上形成孔的电穿孔已用于细胞的转化和灭菌。在本文中，我们报道了使用高电场脉冲施加在大肠杆菌和啤酒酵母中提取核酸的方法。样品为1.0×10–3 mol / L NaCl水溶液，其中包含大肠杆菌和酿酒酵母。在室温下向样品施加高压脉冲。这些结果显示如下：（1）当对大肠杆菌和酿酒酵母施加4〜13kV / mm的脉冲电场时，估计的核酸量随施加的脉冲电场强度的增加而增加。（2）在大肠杆菌的情况下，在5 kV / mm或更高的电场下确认2–3 kbp的带，在8 kV / mm或更高的电场下确认10 kbp的带。（3）在酿酒酵母的情况下，在9 kV / mm或更高的电场下可确认2–3 kbp的带。如果在13 kV / mm以下，则无法确认10 kbp的频带。