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Atomic force microscopy studies of enamel, inner enamel, dentin, and cementum in canine teeth
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-01-06 , DOI: 10.1002/jemt.23668
Shahram Solaymani 1, 2 , Negin Beryani Nezafat 1 , Ştefan Ţălu 3 , Azizollah Shafiekhani 4 , Vali Dalouji 5 , Ali Amiri 6 , Sahar Rezaee 2 , Ilya A Morozov 7
Affiliation  

The main goal of the present work is to explore the three dimensional (3-D) atomic force microscopy (AFM) images of human teeth and investigating their micromorphology. For this purpose, 10 fresh and permanent canine teeth were selected from a group of 40-year-old men who were candidate for the experimental processes. Afterward, they were all applied for studying the morphology of their hard tissues. The tapping mode of AFM was used to characterize the surface micromorphology on the square areas of 1 μm × 1 μm (512 × 512 pts). AFM results and surface stereometric analysis indicate the relationships between the micromorphology of the surface and the structural properties of these tissues across the length scales. As can be seen, the surface of cementum has the most irregular topography (D = 2.87 ± 0.01) while the most regular topography (D = 2.43 ± 0.01) is found in dentin. Furthermore, the more and less regularity of the surface have been found in inner enamel (Sq = 26.26 nm) and dentin (Sq = 41.28 nm), respectively. Stereometric and fractal analyses give valuable information about human canine teeth via 3-D micromorphology.

中文翻译:

犬牙釉质、内釉质、牙本质和牙骨质的原子力显微镜研究

目前工作的主要目标是探索人类牙齿的三维 (3-D) 原子力显微镜 (AFM) 图像并研究它们的微形态。为此,从一组 40 岁的男性中选出了 10 颗新鲜和恒久的犬齿,这些男性是实验过程的候选者。随后,他们都被用于研究其硬组织的形态。AFM 的轻敲模式用于表征 1 μm × 1 μm(512 × 512 pts)正方形区域的表面微形态。AFM 结果和表面立体测量分析表明表面的微形态与这些组织在整个长度尺度上的结构特性之间的关系。可以看出,牙骨质表面具有最不规则的地形(D = 2.87 ± 0.01) 而最规则的地形 ( D  = 2.43 ± 0.01) 是在牙本质中发现的。此外,分别在内部牙釉质(Sq = 26.26 nm)和牙本质(Sq = 41.28 nm)中发现了越来越多的表面规则性。立体和分形分析通过 3-D 微形态学提供有关人类犬齿的宝贵信息。
更新日期:2021-01-06
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