The fabrication of 3D fibrous scaffolds with highly interconnected pores has been crucial in the development of tissue regeneration techniques. The present study describes the fabrication of 3D fibrous scaffolds by freeze-drying of polydopamine (PDA) coated centrifugal spun gelatin fibers. We wanted to combine the mussel-inspired chemistry, Maillard reaction, and the 3D microstructural advantages of centrifugal spun fibers to develop the green fibrous scaffolds at low cost, high speed, and desired mold shape. The resultant PDA-gelatin fibers exhibited a smooth 3D microstructure with a uniform formation of PDA thin ad-layer that enhanced the mechanical properties and stability of the scaffolds, and thereby decreased the degradation rate. All scaffolds showed promising properties including good dimensional and mechanical stability under wet state, optimal porosity over 94%, and high water uptake of approximately 1500%. The results of cell culture studies, further confirmed that all scaffolds exhibited appropriate biocompatibility, cell proliferation, migration, and infiltration. Particularly, the PDA-coated scaffolds showed a significant enhancement in proliferation, migration, and infiltration of HDF-GFP⁺ cells. These results show that a 3D porous fibrous scaffold with simplifying tunable density and desirable shape on a large scale can be readily prepared for different fields of tissue engineering applications.

具有高度互连的孔的3D纤维支架的制造对于组织再生技术的发展至关重要。本研究描述了通过冷冻干燥聚多巴胺（PDA）涂覆的离心纺丝明胶纤维制造3D纤维支架的方法。我们希望结合贻贝启发的化学，美拉德反应和离心纺丝纤维的3D微结构优势，以低成本，高速度和所需模具形状开发绿色纤维状支架。所得的PDA-明胶纤维表现出平滑的3D微观结构，并具有均匀形成的PDA薄广告层，从而增强了支架的机械性能和稳定性，从而降低了降解速率。所有脚手架均显示出良好的性能，包括在湿态下具有良好的尺寸和机械稳定性，最佳孔隙率超过94％，高吸水率约为1500％。细胞培养研究的结果进一步证实，所有支架均表现出适当的生物相容性，细胞增殖，迁移和浸润。特别是，用PDA涂覆的支架在HDF-GFP的增殖，迁移和浸润方面显示出显着增强⁺细胞。这些结果表明，可以容易地为组织工程应用的不同领域制备具有简化的可调密度和大规模所需形状的3D多孔纤维支架。