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Production of lipids by Chaetoceros affinis in media based on palm oil mill effluent
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2021-01-06 , DOI: 10.1016/j.jbiotec.2020.12.021
Karthick Murugan Palanisamy 1 , Primilla Paramasivam 1 , Gaanty Pragas Maniam 2 , Mohd Hasbi Ab Rahim 2 , Natanamurugaraj Govindan 2 , Yusuf Chisti 3
Affiliation  

Biomass and lipid production by the marine diatom Chaetoceros affinis were characterized under continuous light with aeration. Media based on palm oil mill effluent (POME; 10, 20 and 30 % v/v in distilled water) were used together with a standard control medium. The maximum biomass concentration on day 12 of batch cultures in control medium was 821 ± 71 mg L–1. Under identical conditions, in the best POME medium (20 % POME v/v in distilled water with other inorganic components), the biomass concentration was reduced by ∼11 % to 734 ± 66 mg L–1. The lipid content of the biomass grown in the control medium was 50.8 ± 4.5 % by dry weight, but was a little lower (48.9 ± 4.1 % by dry wt) in the above specified best POME medium. In the best POME medium, oleic acid was the major fatty acid (72.3 ± 5.2 % by weight) in the total lipids extracted from the biomass and monounsaturated fatty acids were the main type of fatty acids (74.6 ± 5.2 %). POME levels of >20 % in the medium suppressed both biomass and lipid production relative to the medium with 20 % POME.



中文翻译:

在基于棕榈油厂流出物的培养基中通过 Chaetoceros affinis 生产脂质

海洋硅藻Chaetoceros affinis 的生物质和脂质生产在连续光照和曝气下进行了表征。基于棕榈油厂流出物的培养基(POME;蒸馏水中的 10、20 和 30% v/v)与标准对照培养基一起使用。在对照培养基中分批培养第 12 天的最大生物量浓度为 821 ± 71 mg L –1。在相同条件下,在最佳 POME 培养基中(20 % POME v/v 与其他无机成分的蒸馏水),生物量浓度降低了约 11% 至 734 ± 66 mg L –1. 在对照培养基中生长的生物质的脂质含量为 50.8 ± 4.5 % 干重,但在上述指定的最佳 POME 培养基中略低(48.9 ± 4.1 % 干重)。在最好的 POME 培养基中,油酸是从生物质中提取的总脂质中的主要脂肪酸(72.3 ± 5.2 % 重量),单不饱和脂肪酸是脂肪酸的主要类型(74.6 ± 5.2 %)。相对于含有 20% POME 的培养基,培养基中 >20% 的 POME 水平抑制了生物量和脂质的产生。

更新日期:2021-01-14
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