Qualitative screening of prohibited drugs in dietary supplements based on a miniature homemade mass spectrometer was presented, and its application for real health sample analyses was demonstrated in this work. To alleviate the labor and equipment-intensive procedures of traditional detection methods, simplified sample preparation procedures were coupled with one-time use Nano electrospray ionization sources for practical sample analyses. A small tandem mass spectrometry database was built for 16 illegal adulterants, so that these prohibited drugs could be quickly screened in different samples. Tandem mass spectrometry results were compared with a commercial orbitrap mass spectrometer. After exploring some possible factors, such as the sonication duration, the reproducibility of the method and the effect of matrix on sensitivity, the instrument and dataset were then used for the screening of these 16 adulterants in five commercial products: one weight loss product and four products claiming to strengthen immunity. Illicit adulterants were detected in the weight loss product but not in other samples. These results were also confirmed using the conventional liquid chromatography-mass spectrometry technique.

提出了基于微型自制质谱仪对膳食补充剂中违禁药物进行定性筛选的方法，并证明了其在实际健康样品分析中的应用。为了减轻传统检测方法的劳动和设备密集型程序，简化的样品制备程序与一次性使用的纳米电喷雾电离源相结合，可用于实际样品分析。为16个非法掺假者建立了一个小的串联质谱数据库，以便可以在不同样品中快速筛选这些违禁药物。将串联质谱分析结果与商用orbitrap质谱仪进行比较。探索了一些可能的因素，例如超声处理的持续时间，方法的重现性以及基质对灵敏度的影响，然后，该仪器和数据集用于筛选五种商业产品中的这16种掺杂物：一种减肥产品和四种声称具有增强免疫力的产品。在减肥产品中检出了非法掺假品，但在其他样品中未检出。使用常规液相色谱-质谱技术也证实了这些结果。