P. digitatum, the causative agent of green mold, is one of the most destructive pathogens in the citrus industry. To facilitate basal researches on this important plant pathogen, here we report a finished genome sequence for P. digitatum strain PDW03 using a combination of Illumina, PacBio, and Hi-C sequencing technologies. The assembly comprised 6 chromosomes from telomere to telomere and encodes approximately 9000 proteins. Genomic re-analyses identified 302 Carbohydrate-active enzymes, 420 secreted proteins, and 39 secondary metabolite (SM) gene clusters. Furthermore, we found 10 fragmentary SM clusters in the P. digitatum PDW03 genome. Pangenome analysis based on 5 P. digitatum genomes available showed that conserved orthogroups account for ~68% of the species pangenome. Taken together, this fully completed P. digitatum genome will provide an optimum resource for further researches to investigate the driving forces of fungal host switch and effectors functioning in plant-pathogen interaction.

P. digitatum是绿色霉菌的病原体，是柑橘产业中最具破坏性的病原体之一。为了促进对这一重要植物病原体的基础研究，我们在此报告了使用 Illumina、PacBio 和 Hi-C 测序技术的P. digitatum菌株 PDW03 的完整基因组序列。该组件包括从端粒到端粒的 6 条染色体，并编码大约 9000 种蛋白质。基因组重新分析确定了 302 种碳水化合物活性酶、420 ​​种分泌蛋白和 39 种次级代谢物 (SM) 基因簇。此外，我们在P. digitatum PDW03 基因组中发现了 10 个片段的 SM 簇。基于 5 P. digitatum的泛基因组分析可用的基因组显示保守的邻位群占物种泛基因组的约 68%。总之，这个完整的P. digitatum基因组将为进一步研究提供最佳资源，以研究真菌宿主开关和在植物-病原体相互作用中起作用的效应子的驱动力。