Polymethoxyflavones (PMF) are a group of flavonoids isolated from citrus peels that show crystallization properties. The purpose of this study was to investigate the impact of PMF crystallization on the microstructure, stability and bioaccessibility of PMF-loaded high internal phase emulsions (HIPE) stabilized using protein and polysaccharide complexes. To improve the loading capacity of PMF, a HIPE stabilized using biopolymeric complexes was developed as a container for PMF at concentrations ranging from 1 to 4%. At supersaturation, low-density crystals remained dispersed throughout the HIPE while high-density crystals formed a three-dimensional network that disrupted the oil-in-water interface. Based on the microstructure and the rheological studies, the density of the crystals affected the stability properties. The storage temperature significantly influenced the stability of the PMF-containing HIPE system. The simulated lipolysis study showed that the bioaccessibility of crystalline PMF in a HIPE stabilized using protein-polysaccharide complexes was lower than that of solubilized PMF, indicating that the dissolution of PMF could significantly affect their bioaccessibility with simulated digestion conditions. To conclude, crystal formation in a lipid-rich delivery system affected the stability during storage, as well as the dissolution with simulated digestion condition.

聚甲氧基黄酮（PMF）是从柑橘类果皮中分离出来的一组类黄酮，具有结晶特性。这项研究的目的是调查PMF结晶对使用蛋白质和多糖复合物稳定的PMF负载的高内相乳液（HIPE）的微观结构，稳定性和生物可及性的影响。为了提高PMF的负载能力，开发了一种使用生物聚合物复合物稳定的HIPE作为PMF的容器，其浓度范围为1-4％。在过饱和状态下，低密度晶体保持分散在整个HIPE中，而高密度晶体形成三维网络，破坏了水包油界面。基于微观结构和流变学研究，晶体的密度影响稳定性。储存温度显着影响了含PMF的HIPE系统的稳定性。模拟脂解研究表明，在使用蛋白质-多糖复合物稳定的HIPE中，结晶PMF的生物利用度低于溶解的PMF，这表明在模拟消化条件下，PMF的溶解会显着影响其生物利用度。总而言之，富含脂质的递送系统中的晶体形成会影响储存过程中的稳定性以及在模拟消化条件下的溶出度。表明在模拟消化条件下，PMF的溶解会显着影响其生物利用度。总而言之，富含脂质的递送系统中的晶体形成会影响储存过程中的稳定性以及在模拟消化条件下的溶出度。表明在模拟消化条件下，PMF的溶解会显着影响其生物利用度。总而言之，富含脂质的递送系统中的晶体形成会影响储存过程中的稳定性以及在模拟消化条件下的溶出度。