Fusarium dry rot affects up to 60% of potato tubers during storage. Pathogen resistance development to synthetic chemical fungicides is a main contributing factor. Previous work has demonstrated the use of bacterial antagonists against plant pathogens. In this study, bacteria isolated from disease suppressive composts were tested to evaluate their antagonistic activity against F. sambucinum in vitro, as well as to assess their ability to reduce Fusarium dry rot of potato tubers. To identify the potential implication of antifungal compounds in their inhibitory activity, bacterial extracts were produced and assayed against F. sambucinum mycelial growth and conidial germination. In vitro results showed that all tested bacteria suppressed F. sambucinum mycelial growth over six days of incubation. When testing the bacteria against potato dry rot, 30 of 32 bacteria reduced the symptoms of the disease at the higher bacterial concentration tested. The combined in vitro and in vivo studies indicated Bacillus subtilis B9–8 , Pseudomonas moraviensis F9–6, Pseudomonas koreensis F9–9, and Pseudomonas gessardii M9–16 were the most effective strains. Extracts from these four bacteria were able to inhibit mycelial growth and/or conidial germination of the fungal mold. Results from this study suggests that multiple antagonistic bacteria may be useful in controlling Fusarium dry rot on potato tubers. Extracts from the four most effective bacteria indicated that antibiosis was a main mode of action against F. sambucinum .

中文翻译：

---

使用有益细菌处理抑制马铃薯枯萎病

在储存期间，镰刀菌干腐病影响多达 60% 的马铃薯块茎。病原体对合成化学杀菌剂的抗性发展是一个主要的促成因素。以前的工作已经证明了使用细菌拮抗剂来对抗植物病原体。在这项研究中，对从疾病抑制堆肥中分离的细菌进行了测试，以评估它们在体外对接骨木的拮抗活性，以及​​评估它们减少马铃薯块茎镰刀菌干腐病的能力。为了确定抗真菌化合物对其抑制活性的潜在影响，生产了细菌提取物，并针对接骨木菌丝体生长和分生孢子萌发进行了分析。体外结果表明，所有测试的细菌在孵育六天后都能抑制接骨木菌丝体的生长。当测试细菌对抗马铃薯干腐病时，在测试的较高细菌浓度下，32 种细菌中有 30 种减轻了疾病的症状。体外和体内联合研究表明，枯草芽孢杆菌 B9-8、莫拉维假单胞菌 F9-6、韩国假单胞菌 F9-9 和绿脓杆菌 M9-16 是最有效的菌株。这四种细菌的提取物能够抑制真菌霉菌的菌丝体生长和/或分生孢子萌发。这项研究的结果表明，多种拮抗细菌可能有助于控制马铃薯块茎上的镰刀菌干腐病。四种最有效细菌的提取物表明，抗菌作用是对抗 F. sambucinum 的主要作用方式。Pseudomonas moraviensis F9-6、Pseudomonas koreensis F9-9 和 Pseudomonas gessardii M9-16 是最有效的菌株。这四种细菌的提取物能够抑制真菌霉菌的菌丝体生长和/或分生孢子萌发。这项研究的结果表明，多种拮抗细菌可能有助于控制马铃薯块茎上的镰刀菌干腐病。四种最有效细菌的提取物表明，抗菌作用是对抗 F. sambucinum 的主要作用方式。Pseudomonas moraviensis F9-6、Pseudomonas koreensis F9-9 和 Pseudomonas gessardii M9-16 是最有效的菌株。这四种细菌的提取物能够抑制真菌霉菌的菌丝体生长和/或分生孢子萌发。这项研究的结果表明，多种拮抗细菌可能有助于控制马铃薯块茎上的镰刀菌干腐病。四种最有效细菌的提取物表明，抗菌作用是对抗 F. sambucinum 的主要作用方式。