Astrocytes are an early and important target of Zika virus (ZIKV) infection in the developing brain, but the impacts of infection on astrocyte function remain controversial. Given that nonhuman primate (NHP) models of ZIKV infection replicate aspects of neurologic disease seen in human infections, we cultured primary astrocytes from the brain tissue of infant rhesus macaques and then infected the cells with Asian or African lineage ZIKV to identify transcriptional patterns associated with infection in these cells. The African lineage virus appeared to have greater infectivity and promote stronger antiviral signaling, but infection by either strain ultimately produced typical virus response patterns. Both viruses induced hypoxic stress, but the Asian lineage strain additionally had an effect on metabolic and lipid biosynthesis pathways. Together, these findings describe an NHP astrocyte model that may be used to assess transcriptional signatures following ZIKV infection.

星形胶质细胞是发育中大脑中寨卡病毒（ZIKV）感染的早期重要目标，但感染对星形胶质细胞功能的影响仍然存在争议。鉴于 ZIKV 感染的非人灵长类 (NHP) 模型复制了人类感染中所见的神经系统疾病的各个方面，我们从恒河猴幼年的脑组织中培养原代星形胶质细胞，然后用亚洲或非洲谱系 ZIKV 感染细胞，以识别与 ZIKV 相关的转录模式。这些细胞的感染。非洲谱系病毒似乎具有更强的传染性并促进更强的抗病毒信号传导，但任一毒株的感染最终都会产生典型的病毒反应模式。两种病毒都会引起缺氧应激，但亚洲谱系菌株还对代谢和脂质生物合成途径产生影响。总之，这些发现描述了 NHP 星形胶质细胞模型，可用于评估 ZIKV 感染后的转录特征。