Glioblastoma (GBM) is one of the most lethal tumor of all human cancers. Due to its poor response to chemotherapy and radiotherapy as well as its high rate of recurrence after treatment, the treatment is still undesired. The identification of potential related genes and bio-markers in the development of GBM could provide some new targets for the treatment of GBM. Our purpose in this study was to evaluate the mission of COL8A2 in GBM. Combined with TCGA, Oncomine databases, CGGA, GEPIA website and qRT-PCR analyses, we found that COL8A2 was up-regulated both in GBM tissues and cells compared to the controls. Moreover, the high COL8A2 expression was associated with the shorter overall survival of patients with GBM. The expression of COL8A2 was also positively correlated with metastasis-associated genes including vimentin, snail, slug, MMP2 and MMP7 according to GEPIA website. Knockdown of COL8A2 could suppress the cell proliferation, cell migration and invasion, whereas the overexpression of COL8A2 significantly expedited these processes. What’s more, the outcome of western blot analysis manifested that COL8A2 could induced the expression of vimentin, snail, slug, MMP2 and MMP7. Taken together, COL8A2 activated cell proliferation, cell migration and invasion via raising the relative expression of EMT-related proteins in GBM. Therefore, our investigation suggests the oncogenic role of COL8A2 in GBM and provides a potential application of COL8A2 for GBM therapy.

胶质母细胞瘤 (GBM) 是所有人类癌症中最致命的肿瘤之一。由于其对化疗和放疗反应差，治疗后复发率高，治疗效果仍不理想。鉴定GBM发生发展中潜在的相关基因和生物标志物可为GBM的治疗提供一些新的靶点。我们在这项研究中的目的是评估 COL8A2 在 GBM 中的任务。结合 TCGA、Oncomine 数据库、CGGA、GEPIA 网站和 qRT-PCR 分析，我们发现与对照相比，COL8A2 在 GBM 组织和细胞中均上调。此外，高 COL8A2 表达与 GBM 患者较短的总生存期相关。COL8A2 的表达也与转移相关基因呈正相关，包括波形蛋白、蜗牛、蛞蝓、根据 GEPIA 网站的 MMP2 和 MMP7。COL8A2 的敲低可以抑制细胞增殖、细胞迁移和侵袭，而 COL8A2 的过表达显着加速了这些过程。此外，蛋白质印迹分析结果表明，COL8A2可以诱导波形蛋白、蜗牛、蛞蝓、MMP2和MMP7的表达。总之，COL8A2 通过提高 GBM 中 EMT 相关蛋白的相对表达来激活细胞增殖、细胞迁移和侵袭。因此，我们的研究表明 COL8A2 在 GBM 中的致癌作用，并提供了 COL8A2 在 GBM 治疗中的潜在应用。Western Blot分析结果表明COL8A2可以诱导vimentin、snail、slug、MMP2和MMP7的表达。总之，COL8A2 通过提高 GBM 中 EMT 相关蛋白的相对表达来激活细胞增殖、细胞迁移和侵袭。因此，我们的研究表明 COL8A2 在 GBM 中的致癌作用，并提供了 COL8A2 在 GBM 治疗中的潜在应用。Western Blot分析结果表明COL8A2可以诱导vimentin、snail、slug、MMP2和MMP7的表达。总之，COL8A2 通过提高 GBM 中 EMT 相关蛋白的相对表达来激活细胞增殖、细胞迁移和侵袭。因此，我们的研究表明 COL8A2 在 GBM 中的致癌作用，并提供了 COL8A2 在 GBM 治疗中的潜在应用。