The African ginger (Mondia whitei) is an endangered, endemic African medicinal plant, widely used as an aphrodisiac and to manage gastrointestinal problems and anorexia. Due to high demand, it is over-utilized from the wild without replenishment and destroyed by inappropriate harvesting methods. Therefore, there is a need to develop an efficient conservation system for the African ginger. This study focused on developing an in vitro propagation procedure for the African ginger through indirect shoot organogenesis. Optimal callus formation was induced from leaf explants cultured on Murashige and Skoog (MS) basal medium fortified with 1.5 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). The 1.5 mg L⁻¹ 6-benzylaminopurine (BAP) concentration was optimal with 50% of the callus-forming buds. For shoot formation, media with 2.0 mg L⁻¹ BAP and 1.0 mg L⁻¹ NAA resulted in 60% of the buds developing shoots. Regenerated shoots were rooted on woody plant medium (WPM) fortified with 0.1 mg L⁻¹ BAP and no rooting hormone included. The survival rate of weaned and acclimatized plants was 70% and up to 80% respectively. The study demonstrates an in vitro propagation route for the African ginger which could support its mass production for medicinal use, availing of planting materials, conservation, and commercialization purposes.

非洲生姜（Mondia whitei）是一种濒临灭绝的地方性非洲药用植物，被广泛用作壮阳药并用于治疗胃肠道疾病和厌食症。由于需求量大，在没有补给的情况下从野外过度利用，并被不适当的收割方法破坏。因此，需要开发一种有效的非洲生姜保护系统。这项研究的重点是通过间接芽器官发生为非洲生姜开发体外繁殖程序。从在以1.5 mg L ^-1 2,4-二氯苯氧基乙酸（2,4-D）强化的Murashige和Skoog（MS）基础培养基上培养的叶片外植体诱导最佳的愈伤组织形成。1.5 mg L ^-1对于50％的愈伤组织芽，6-苄氨基嘌呤（BAP）浓度最佳。对于芽形成，具有2.0 mg L ^-1 BAP和1.0 mg L ^-1 NAA的培养基导致60％的芽发育为芽。再生的芽生根于强化有0.1 mg L ^-1 BAP且不包含生根激素的木本植物培养基（WPM）。断奶和驯化植物的存活率分别为70％和高达80％。该研究表明了非洲生姜的体外繁殖途径，可以支持其大量生产，以供药用，提供种植材料，进行保护和商业化。