Assessment of bacterial growth is an essential tool in many fields of study. Numerous methods are available for that purpose, but they are not without disadvantages. The present study aimed to investigate the application of a water soluble oxygen sensor [Ru(dpp(SO₃Na)₂)₃]Cl₂x6H₂O (RuBPS) in physiological and biochemical studies of Pectobacterium spp. and other bacteria in 96-well microplate systems. The method was optimized with respect to compound concentration, microplate type, and measurement parameters. The growth of bacteria was assessed under increased salinity, acidic and alkaline pH, decreased water availability, and osmotic stress. Utilization of various sugars by bacteria as the only carbon source and the usefulness of RuBPS in antibiotic susceptibility assays were also investigated. The studies confirmed that the respirometry assay can be successfully used to assess bacterial growth in place of the optical density measurement and the resazurin assay, or in conjugation with those methods. The assay is of special value when growth media are non-homogeneous, bacteria produce reductive compounds during growth, or when bacteria do not grow well in the medium of choice. It may be used to study adaptation potential of plant pathogenic bacteria and other bacteria, and their possible spread into new ecological niches.

在许多研究领域中，细菌生长的评估是必不可少的工具。有许多方法可用于该目的，但它们并非没有缺点。本研究旨在探讨水溶性氧传感器[Ru（dpp（SO ₃ Na）₂）₃ ] Cl ₂ x6H ₂ O（RuBPS）在果胶杆菌生理生化研究中的应用。spp。以及96孔微孔板系统中的其他细菌。在化合物浓度，微孔板类型和测量参数方面对该方法进行了优化。在盐度增加，酸性和碱性pH值增加，水利用量减少和渗透胁迫下评估了细菌的生长。还研究了细菌作为唯一碳源对各种糖的利用以及RuBPS在抗生素药敏试验中的有用性。研究证实，呼吸测定法可以代替光密度测量法和刃天青测定法，或与这些方法结合，可成功用于评估细菌的生长。当生长培养基不均匀，细菌在生长过程中产生还原性化合物或细菌在所选培养基中生长不好时，该测定法具有特殊价值。