Cell-assisted lipotransfer (CAL) is an advanced lipoinjection method that uses autologous lipotransfer with addition of a stromal vascular fraction (SVF) containing adipose-derived stromal stem cells (ASCs). The CAL procedure of manual isolation of cells from fat requires cell processing to be performed in clean environment. To isolate cells from fat without the need for a cell processing center, such as in a procedure in an operation theater, we developed a novel method for processing SVF using a closed cell washing concentration device (CCD) with a hollow fiber membrane module. The CCD consists of a sterilized closed circuit, bags and hollow fiber, semi-automatic device and the device allows removal of >99.97% of collagenase from SVF while maintaining sterility. The number of nucleated cells, ASCs and viability in SVF processed by this method were equivalent to those in SVF processed using conventional manual isolation. Our results suggest that the CCD system is as reliable as manual isolation and may also be useful for CAL. This approach will help in the development of regenerative medicine at clinics without a cell processing center.

细胞辅助脂肪移植 (CAL) 是一种先进的脂肪注射方法，它使用自体脂肪移植并添加含有脂肪来源的基质干细胞 (ASC) 的基质血管部分 (SVF)。从脂肪中手动分离细胞的 CAL 程序需要在干净的环境中进行细胞处理。为了在不需要细胞处理中心的情况下从脂肪中分离细胞，例如在手术室的程序中，我们开发了一种使用带有中空纤维膜模块的闭式细胞洗涤浓缩装置 (CCD) 处理 SVF 的新方法。CCD 由灭菌封闭回路、袋子和中空纤维、半自动装置组成，该装置允许从 SVF 中去除 >99.97% 的胶原酶，同时保持无菌。有核细胞的数量，通过这种方法处理的 SVF 中的 ASC 和活力与使用传统手动隔离处理的 SVF 中的相同。我们的结果表明 CCD 系统与手动隔离一样可靠，也可能对 CAL 有用。这种方法将有助于在没有细胞处理中心的诊所开发再生医学。