Plants grown in stony soils have better developed root systems and higher crop yields. The roles of physical and chemical effects of stony soils on plant growth have been published, but the roles of soil microbiota have not been investigated. In this study, Tetrastigma hemsleyanum plants were cultivated for 2 years in stony soils and the same soils after removing rock fragments (non-stony soils). The composition of bacterial community and the tuber transcriptome were analyzed, using multiple bioinformatics methods. Compared with non-stony soils, stony soils supported a significantly different bacterial community, which was associated with larger tuber size of T. hemsleyanum. Stony soils had greater bacterial diversity, co-occurrence network complexity, and greater abundance of bacterial taxa belonging to Actinobacteria, Rokubacteria, Rhizobiales, Desulfarculaceae, and Chthoniobacteraceae than the corresponding microbiota in non-stony soils. The differential soil bacterial communities between stony and non-stony soils may be mainly driven by soil physicochemical properties, such as available S, organic matter, and pH. The discriminatory bacterial taxa of soils shaped the bacterial communities of rhizosphere soil, and tuber surface soil (TS), which was strongly correlated with tuber size parameters. In addition, the potential functions of the discriminatory bacterial taxa in TS corresponded closely with gene pathways of the host, like phytohormone biosynthesis, photosynthesis, and biotic stress resistance, which are crucial for the initiation and development of tubers. These results not only help us to better understand the role of stony soils in improving plant growth but also provide a reference for increasing tuber yield through the use of microbial inocula.

在石质土壤中生长的植物具有更好的根系发育和更高的农作物产量。石质土壤对植物生长的物理和化学作用的作用已被发表，但土壤微生物群的作用尚未得到研究。在这项研究中，去除石块后（非石壤土），在石质土壤和相同的土壤中种植了四叶草（Tetrastigma hemsleyanum）植物。使用多种生物信息学方法分析了细菌群落的组成和块茎转录组。与非石质土壤相比，石质土壤具有明显不同的细菌群落，这与棉铃虫的块茎较大有关。。与非石质土壤中相应的微生物群落相比，石质土壤具有更大的细菌多样性，共现网络复杂性，并且属于放线菌属，Rokubacteria，根瘤菌，Desulfarculaceae和Chthoniobacteraceaceae的细菌类群丰富。石质土壤和非石质土壤之间不同的土壤细菌群落可能主要由土壤的理化特性（例如有效硫，有机质和pH）驱动。土壤的歧视性细菌类群塑造了根际土壤和块茎表层土壤（TS）的细菌群落，这与块茎大小参数密切相关。此外，TS中歧视性细菌类群的潜在功能与宿主的基因途径密切相关，例如植物激素的生物合成，光合作用和生物胁迫抗性，这对于块茎的萌芽和发育至关重要。这些结果不仅帮助我们更好地了解石质土壤在改善植物生长中的作用，而且还为通过使用微生物接种提高块茎产量提供了参考。