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Biotechnological strategies for the sustainable production of diosgenin from Dioscorea spp.
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2021-01-06 , DOI: 10.1007/s00253-020-11055-3
Romaan Nazir , Vijay Kumar , Suphala Gupta , Padmanabh Dwivedi , Devendra Kumar Pandey , Abhijit Dey

Abstract

Diosgenin is a plant-derived secondary metabolite mainly present in the members of the plant family Dioscoreaceae. It is a pharmaceutically important compound because of its anti-cancerous, anti-diabetic, anticoagulant, anti-thrombosis, anti-inflammatory, anti-viral, anti-ageing and other properties. Biotechnology provides an opportunity to genetically manipulate cells, tissues, organs or the whole organisms by propagating them in vitro in order to harvest the bioactive compounds. Diosgenin production from botanical sources is being improved by in vitro techniques which include elicitation, genetic transformations and bioconversions. Various techniques have been developed to obtain compounds for drug detection including separation from plants and other natural sources, molecular modelling, synthetic chemistry and combinatorial chemistry. Development in molecular markers determines genetic relationship, genetic linkage map construction, genetic diversity and identification. For rapid clonal propagation and ex situ conservation, the in vitro tools involving plant cell, tissue and organ culture have been well documented for plant-derived diosgenin production. The present review encompasses the wide application of the biotechnological techniques for diosgenin production via elucidating its biosynthetic pathway, in vitro production and mass propagation and elicitation. In addition, molecular marker-mediated diversity assessment of diosgenin containing plant species is also discussed. The review also presents the recent literature to explore the limitations of the relevant studies and future direction of research on production of diosgenin from Dioscorea spp.

Key points

• Critical and updated assessment on sustainable production of diosgenin from Dioscorea spp.

In vitro propagation of Dioscorea spp. and elicitation of diosgenin production.

• Diversity assessment of Dioscorea spp. using molecular markers.

Graphical abstract



中文翻译:

薯Di可持续生产薯gen皂甙元的生物技术策略。

摘要

薯gen皂素是植物来源的次生代谢产物,主要存在于薯os科的成员中。由于其具有抗癌,抗糖尿病,抗凝血,抗血栓形成,抗炎,抗病毒,抗衰老和其他特性,因此是药学上重要的化合物。生物技术通过在体外繁殖以收获生物活性化合物,提供了遗传控制细胞,组织,器官或整个生物的机会。来自植物来源的薯os皂素的生产正在通过体外技术进行改进,这些技术包括诱导,遗传转化和生物转化。已经开发出各种技术来获得用于药物检测的化合物,包括从植物和其他天然来源中分离,分子模拟,合成化学和组合化学。分子标记的发展决定了遗传关系,遗传连锁图谱的构建,遗传多样性和鉴定。对于快速的克隆繁殖和非原位保存,涉及植物细胞,组织和器官培养的体外工具已被充分证明可用于植物来源的薯os皂苷元的生产。本综述涵盖了薯techniques皂甙元生产的生物技术技术的广泛应用,方法是阐明薯s皂甙元的生物合成途径,体外生产以及大量繁殖和诱导。此外,还讨论了含薯os皂苷元的植物物种的分子标记介导的多样性评估。该综述还提出了最新文献,以探讨相关研究的局限性以及从中生产薯os皂苷元的研究方向。组织和器官培养已被充分证明可以用于植物来源的薯os皂苷元的生产。本综述涵盖了薯techniques皂甙元生产的生物技术技术的广泛应用,方法是阐明薯s皂甙元的生物合成途径,体外生产以及大量繁殖和诱导。此外,还讨论了含薯os皂苷元的植物物种的分子标记介导的多样性评估。该综述还提出了最新文献,以探讨相关研究的局限性以及从中生产薯os皂苷元的研究方向。组织和器官培养已被充分证明可以用于植物来源的薯os皂苷元的生产。本综述涵盖了薯techniques皂甙元生产的生物技术技术的广泛应用,方法是阐明薯s皂甙元的生物合成途径,体外生产以及大量繁殖和诱导。此外,还讨论了含薯os皂苷元的植物物种的分子标记介导的多样性评估。该综述还提出了最新文献,以探讨相关研究的局限性以及从中生产薯os皂苷元的研究方向。此外,还讨论了含薯os皂苷元的植物物种的分子标记介导的多样性评估。该综述还提出了最新文献,以探讨相关研究的局限性以及从中生产薯os皂苷元的研究方向。此外,还讨论了含薯os皂苷元的植物物种的分子标记介导的多样性评估。该综述还提出了最新文献,以探讨相关研究的局限性以及从中生产薯os皂苷元的研究方向。薯os属。

关键点

•对薯Di薯sustainable中薯production皂甙元可持续生产的重要和最新评估。

薯os的体外繁殖。和诱导薯os皂素的生产。

•薯Di的多样性评估。使用分子标记。

图形概要

更新日期:2021-01-06
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