One plant and one to two ratoon crops are the predominant patterns of sugarcane cultivation in sub-tropical part of India. Despite high agricultural inputs, yield of ratoon crop gets dwindled in the subsequent years. The microbial community, particularly bacteria and fungi, in the rhizosphere and their interaction with the root system, in general influences plant productivity. For the present study, an early maturing sugarcane variety (CoLk 94184), was used to establish plant and winter-initiated ratoon crops in 2016–2018. Soils pertaining to both plant and ratoon rhizospheres were subjected to biochemical analysis, microbial DNA isolation and high-throughput sequencing of 16S rRNA genes to assess the microbial diversity and associated characteristics impacting cane yield. Although alpha diversity of bacterial community was observed high in the soils of both plant and ratoon crops, the species richness/diversity was more in plant crop. Bacterial community structure in the rhizosphere of plant crop was predominantly consisted of phyla Actinobacteria (35.68%), Gemmatimonadetes (29.26%), Chloroflexi (26.73%) and Proteobacteria (16.68%), while ratoon rhizosphere revealed dominance of Acidobacteria (20.77%) and Bacteroidetes (10.7%). Though studies revealed the presence of rich bacterial community in the rhizospheres of both plant and ratoon crops of sugarcane, dominance of Acidobacteria and meager proportion of Actinobacteria and Proteobacteria in ratoon crop possibly limited its productivity. Along with high total phenols (7.27 mg/g dry wt), ratoon crop depicted less active root system as revealed by scanning electron microscopy. Dominance of thermophilic bacterial phyla Chloroflexi and Gemmatimonadetes which was observed in sugarcane rhizosphere supports better crop growth in drought. However, management of soil microbial community is required to improve the ratoon crop productivity.

一种植物和一到两种宿根作物是印度亚热带地区甘蔗种植的主要模式。尽管农业投入高，但随后几年宿根作物的产量却在下降。根际微生物群落，尤其是细菌和真菌，以及它们与根系的相互作用，通常会影响植物的生产力。在本研究中，早熟甘蔗品种 (CoLk 94184) 用于在 2016-2018 年建立植物和冬季开始的宿根作物。对属于植物和宿根根际的土壤进行生化分析、微生物 DNA 分离和 16S rRNA 基因的高通量测序，以评估微生物多样性和影响甘蔗产量的相关特征。虽然在植物和宿根作物的土壤中观察到细菌群落的 α 多样性很高，但植物作物的物种丰富度/多样性更多。植物作物根际细菌群落结构主要由门组成Actinobacteria (35.68%), Gemmatimonadetes (29.26%), Chloroflexi (26.73%) 和Proteobacteria (16.68%)，而宿根根际显示出主要是Acidobacteria (20.77%) 和Bacteroidetes (10.7%)。虽然研究表明甘蔗植物和宿根作物的根际都存在丰富的细菌群落，但酸杆菌占主导地位，放线菌和变形菌的比例微乎其微宿根作物可能限制了其生产力。扫描电子显微镜显示，随着总酚含量高（7.27 毫克/克干重），宿根作物的根系活性较低。在甘蔗根际观察到的嗜热细菌门Chloroflexi和Gemmatimonadetes 的优势支持在干旱条件下更好的作物生长。然而，需要对土壤微生物群落进行管理以提高宿根作物的生产力。