Perilla frutescens fruits (PFO) is rich in α-linolenic acid (ALA) and exhibits biological activities. We aimed to investigate analgesic, anti-inflammatory and anti-ulcer activities of PFO and PFO-supplemented soybean milk (PFO-SM) in animal models. Analgesic activity was assessed in acetic acid-induced writhing in mice, while anti-inflammatory activity was performed in ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced hind paw edema in rats. Anti-ulcer effects were conducted in water immersion stress, HCl/ethanol, and indomethacin-induced gastric ulcer in rats. Distinctly, PFO (6.96 mg ALA and 2.61 LA mg equivalent/g) did not induce acute toxicity (LD50 >10 mL/kg) in mice. PFO (2.5 and 5 mL/kg) and PFO-SM (0.05 mL PFO equivalence/kg) inhibited incidences of writhing (16.8, 18 and 32.3%, respectively) in acetic acid-induced mice. In addition, topical applications of PFO (0.1 and 1 mL/ear) significantly inhibited EPP-induced ear edema (59.3 and 65.7%, respectively) in rats, while PFO-SM slightly inhibited ear edema (25.9%). However, PFO and PFO-SM did not inhibit carrageenan-induced hind paw edema in rats. Indeed, PFO (2.5 and 5 mL/kg) significantly inhibited gastric ulcers in rats that induced by water immersion stress (92.4 and 96.6%, respectively), HCl/ethanol (74.8 and 73.3%, respectively) and indomethacin (68.8 and 88.9%, respectively), while PFO-SM did not. PFO displayed potent analgesic, anti-inflammatory and anti-ulcer properties, while PFO-SM exerted only analgesic properties. Thus, Thai perilla fruit oil and its functional drink offers potential benefits in treatment of analgesic, inflammatory diseases and gastric ulcer.

中文翻译：

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泰国紫苏果油在动物体内的镇痛、抗炎和抗溃疡特性。

紫苏果实（PFO）富含α-亚麻酸（ALA），具有生物活性。我们旨在研究 PFO 和 PFO 补充豆奶 (PFO-SM) 在动物模型中的镇痛、抗炎和抗溃疡活性。在醋酸诱导的小鼠扭体中评估镇痛活性，而在苯丙酸乙酯 (EPP) 诱导的大鼠耳水肿和角叉菜胶诱导的后爪水肿中进行抗炎活性。在水浸泡应激、盐酸/乙醇和吲哚美辛诱导的大鼠胃溃疡中进行了抗溃疡作用。显然，PFO（6.96 毫克 ALA 和 2.61 LA 毫克当量/克）不会在小鼠中引起急性毒性（LD50 > 10 毫升/千克）。PFO（2.5 和 5 mL/kg）和 PFO-SM（0.05 mL PFO 当量/kg）抑制了扭体的发生率（16.8%、18% 和 32.3%，分别）在醋酸诱导的小鼠中。此外，局部应用 PFO（0.1 和 1 mL/耳）显着抑制 EPP 诱导的大鼠耳水肿（分别为 59.3% 和 65.7%），而 PFO-SM 轻微抑制耳水肿（25.9%）。然而，PFO 和 PFO-SM 不能抑制角叉菜胶诱导的大鼠后爪水肿。事实上，PFO（2.5 和 5 mL/kg）显着抑制了由浸水应激（分别为 92.4% 和 96.6%）、HCl/乙醇（分别为 74.8% 和 73.3%）和吲哚美辛（分别为 68.8% 和 88.9%）引起的胃溃疡，分别），而 PFO-SM 没有。PFO 显示出有效的镇痛、抗炎和抗溃疡特性，而 PFO-SM 仅具有镇痛特性。因此，泰国紫苏果油及其功能性饮料在治疗镇痛、炎症性疾病和胃溃疡方面具有潜在的益处。