Mammary cancer is a second-most leading cancer among other cancer-related death of women worldwide. In recent years, the medical community has advanced chemotherapies for cancer treatment. However, the survival ratio has not yet been improved. Therefore, we try to identify a novel natural chemopreventive agent for the management of breast cancer. Mangiferin (MGN) is a polyphenol agent; it presents in plants as Gentianaceae and Anacardiaceae families. Our study aimed to investigate inflammation, cell proliferation, and apoptotic molecular mechanisms of MGN on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary cancer. Mammary cancer was induced by a one-time subcutaneous injection of DMBA (0.025 g/rat) in the region of mammary-gland. Herein, we noticed increased tumor-incidence, tumor-volume, reduced antioxidant-enzymes activity, and elevated TBARS levels in plasma and tissues of DMBA alone-induced rats. Further, we noticed an enhanced expression of inflammatory markers NF-κBp65, COX-2, and iNOS, and cell proliferation (PCNA and Cyclin D1), and anti-apoptotic marker (Bcl-2) in DMBA-induced mammary-cancer rats. Our results indicate that oral administration of MGN (100mg/kg b.wt) significantly enhances antioxidants and reduced TBARS levels in DMBA-mediated tumor-bearing rats. Moreover, MGN inhibits NF-κBp65 nucleus transcriptional activation, thereby suppressed inflammation, cell proliferation, and enhanced pro-apoptotic protein in DMBA-induced mammary cancer rats. Furthermore, MGN induced apoptosis was confirmed by TUNEL assay. Collectively, we resolved that MGN impeded DMBA-influenced mammary carcinogenesis through enhance antioxidant, and hinder NF-κB and positive re

中文翻译：

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芒果苷在 DMBA 诱导的乳腺癌发生中具有抑制炎症、细胞增殖并通过抑制 NF-κB 激活促凋亡事件的保护作用


在全球其他与癌症相关的女性死亡中，乳腺癌是第二大癌症。近年来，医学界在癌症治疗方面取得了先进的化学疗法。然而，成活率尚未提高。因此，我们试图寻找一种新型天然化学预防剂来治疗乳腺癌。芒果苷（MGN）是一种多酚剂；它存在于龙胆科和漆树科植物中。我们的研究旨在探讨 MGN 对 7,12-二甲基苯并（a）蒽 (DMBA) 诱导的乳腺癌的炎症、细胞增殖和凋亡分子机制。通过在乳腺区域一次性皮下注射DMBA（0.025g/大鼠）诱导乳腺癌。在此，我们注意到单独 DMBA 诱导的大鼠血浆和组织中肿瘤发生率、肿瘤体积增加、抗氧化酶活性降低以及 TBARS 水平升高。此外，我们注意到 DMBA 诱导的乳腺癌大鼠中炎症标志物 NF-κBp65、COX-2 和 iNOS 以及细胞增殖（PCNA 和 Cyclin D1）以及抗凋亡标志物（Bcl-2）的表达增强。我们的结果表明，口服 MGN（100mg/kg b.wt）可显着增强 DMBA 介导的荷瘤大鼠的抗氧化剂并降低 TBARS 水平。此外，MGN 抑制 NF-κBp65 核转录激活，从而抑制 DMBA 诱导的乳腺癌大鼠的炎症、细胞增殖并增强促凋亡蛋白。此外，通过TUNEL测定证实了MGN诱导的细胞凋亡。总的来说，我们发现 MGN 通过增强抗氧化作用来阻止 DMBA 影响的乳腺癌发生，并阻碍 NF-κB 和正向回复