Rhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications. An extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants Km and Vmax, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na+ (1 and 5 mM) and K+ (5 mM) were promoters of the lipase to enhance its activity with 110, 105.5, and 106.5%, respectively. Chitosan was used as a perfect support for immobilization via both adsorption and cross-linking in which the latter method attained immobilization efficiency of 99.1% and reusability of 12 cycles. The partially purified enzyme proved its ability in forming methyl oleate (biodiesel) through the esterification of oleic acid and transesterification of olive oil. The partially purified and immobilized lipase from Rhizopus oryzae R1 approved excellent efficiency, reusability, and a remarkable role in detergents and biodiesel production.

中文翻译：

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米根霉R1中的脂肪酶：生物柴油生产中的深入表征，固定化和评估

根霉属物种是最著名的脂肪酶生产者之一，其酶适用于许多工业应用。除了评估其用途外，我们的研究重点还在于利用废物生产脂肪酶。使用硫酸铵沉淀从米根霉R1分离株的培养液中部分纯化细胞外脂肪酶至表观均匀性，然后通过透析脱盐。部分纯化的酶是非特异性脂肪酶，在pH 6、40°C下以30分钟的高稳定性记录了最大活性。由Lineweaver-Burk图计算得出的常数Km和Vmax分别为0.3 mg / mL和208.3 U / mL。单价金属离子，例如Na +（1和5 mM）和K +（5 mM）是脂肪酶的启动子，分别以110、105.5和106.5％的比例增强其活性。壳聚糖被用作通过吸附和交联进行固定的完美载体，其中后一种方法的固定效率为99.1％，可重复使用性为12个循环。通过油酸的酯化和橄榄油的酯交换反应，部分纯化的酶证明了其形成油酸甲酯（生物柴油）的能力。米根霉R1的部分纯化和固定化的脂肪酶具有出色的效率，可重复使用性，并且在洗涤剂和生物柴油生产中发挥了重要作用。通过油酸的酯化和橄榄油的酯交换反应，部分纯化的酶证明了其形成油酸甲酯（生物柴油）的能力。米根霉R1的部分纯化和固定化的脂肪酶具有出色的效率，可重复使用性，并且在洗涤剂和生物柴油生产中发挥了重要作用。通过油酸的酯化和橄榄油的酯交换反应，部分纯化的酶证明了其形成油酸甲酯（生物柴油）的能力。米根霉R1的部分纯化和固定化的脂肪酶具有出色的效率，可重复使用性，并且在洗涤剂和生物柴油生产中发挥了重要作用。